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Development of a new HPLC method for rapid histamine quantification in fish and fishery products without sample clean-up

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Abstract

HPLC methods are among the most studied and validated techniques for the quantification of histamine in fish and fishery products; nonetheless, most of these methods require laborious sample extraction and purification. For this purpose, in the present study, an HPLC-FLD method without any sample clean-up procedure was developed, validated and applied for histamine quantification in various fish samples. Histamine was extracted from fish and fishery products samples with perchloric acid; then a simple precolumn derivatization with o-phthalaldehyde was performed in the presence of the reducing agent 2-mercaptoethanol. Once the derivatization was carried out, the separation was performed with a reverse phase HPLC system. In terms of validation, good linearity and specificity over a concentration range from 12.5 to 200 mg/kg were achieved. Also, convenable precision with relative standard deviations smaller than 5.92%, accuracy ranged from 0.40 to 1.84%, and satisfactory recoveries ranged from 100.44 to 101.84%, were obtained. The detection and the quantification limits were calculated at 2.8 and 4.3 mg/kg, respectively. In addition, the effectiveness of the method was evaluated by the analysis of reference materials, proficiency tests and various type of samples. Moreover, the developed method was found to be simple, rapid, and reliable.

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Authors and Affiliations

  1. Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al Malek Essaadi University, Tetouan, Morocco

    Ayoub Kounnoun, Adnane Louajri, Aicha El Baaboua & Hajar Bougtaib

  2. Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Messina, Italy

    Francesco Cacciola

  3. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy

    Luigi Mondello

  4. Chromaleont s.r.l. c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy

    Luigi Mondello

  5. Department of Sciences and Technologies for Human and Environment, University Campus Bio-Medico of Rome, Rome, Italy

    Luigi Mondello

  6. Regional Analysis and Research Laboratory, National Office of Food Safety ONSSA, Tangier, Morocco

    Naoual Alahlah, Meriem Stitou & Mohamed El Maadoudi

Authors
  1. Ayoub Kounnoun
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  2. Adnane Louajri
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  3. Francesco Cacciola
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  4. Aicha El Baaboua
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  5. Luigi Mondello
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  6. Hajar Bougtaib
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  7. Naoual Alahlah
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  8. Meriem Stitou
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  9. Mohamed El Maadoudi
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Correspondence to Ayoub Kounnoun or Francesco Cacciola.

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Kounnoun, A., Louajri, A., Cacciola, F. et al. Development of a new HPLC method for rapid histamine quantification in fish and fishery products without sample clean-up. Eur Food Res Technol 248, 1679–1689 (2022). https://doi.org/10.1007/s00217-022-03995-z

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  • DOI: https://doi.org/10.1007/s00217-022-03995-z

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