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Aptasensor based on fluorescence resonance energy transfer for the determination of kanamycin

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Abstract

Based on fluorescence resonance energy transfer, an ultrasensitive aptasensor was designed to detect kanamycin. Carbon quantum dots (CQDs) connected to the 3′ end of the hairpin aptamer (HP) acted as energy donor, while gold nanoparticles (AuNPs) bound to the 5′ end of HP acted as energy acceptor. The interaction due to the overlapping between absorption spectrum of AuNPs and emission spectrum of CQDs led to an obvious quenching. After adding kanamycin, the specific binding between target and HP made the HP structure change. Then, the distance between CQDs and AuNPs increased gradually, and fluorescence intensity would recover. Therefore, the recovery of fluorescence intensity can be used for kanamycin detection via optical responses. Under optimal conditions, fluorescence intensity had a good linear relationship with the logarithm of kanamycin concentration. The detection limit of kanamycin was 5.7 pM (3.22 × 10–3 μg/kg) and the detection concentration of kanamycin concentration was 0.01–500 nM (5.65 × 10–3–282.80 μg/kg). The aptasensor was also applied into the determination of kanamycin residues in milk.

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Availability of data and materials

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China under Grant (No. 31872909, No. 32001781) and the Natural Science Foundation of Shandong Province under Grant (ZR2017BC001, ZR2020QC249).

Funding

This work was supported by the National Natural Science Foundation of China under Grant (No. 31872909, No. 32001781) and the Natural Science Foundation of Shandong Province under Grant (ZR2017BC001, ZR2020QC249).

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Author notes

  1. Lin Wei and Hui Zhang have contributed equally to this work.

Authors and Affiliations

  1. School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, China

    Lin Wei, Hui Zhang, Xia Sun, Xue Huang, He Li, Falan Li, Yemin Guo & Qingqing Yang

  2. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, China

    Lin Wei, Xia Sun, Falan Li, Yemin Guo & Qingqing Yang

  3. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, 255049, China

    Lin Wei, Xia Sun, Falan Li, Yemin Guo & Qingqing Yang

Authors
  1. Lin Wei
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Contributions

LW: a graduate student. In the group, she undertook the main experimental investigation including sample preparation and measurement and specificity and stability assays and finished part of the data processing and analysis. She finished the first draft and completed the submission and revision of her thesis HZ: a graduate. In the group, she undertook the experimental investigation including the synthesis of AuNPs@HP@CQDs, and also the procedure for the fluorescent detection of KAN. XS: holds a position as a professor at Shandong University of Technology. Her research focuses on biosensors for the safety detection of agricultural products. She finished the data review and proofreading of the paper. Xue Huang, a postdoctor. In the group, he helped finish the preparation of AuNPs and activation of CQDs and the procedure for the fluorescent detection of KAN. He Li, a postdoctor. In the group, he finished buying and processing milk samples and the data review. FL: holds a position as a lecturer at Shandong University of Technology. Her research focuses on detection technology of antibiotic residues in animal food. In the group, she purchased and prepared reagents needed for the experiment. YG: holds a position as a professor at Shandong University of Technology. His research focuses on electrochemical biosensor for the safety detection of agricultural products. He polished this paper. QY: holds a position as an associate professor at Shandong University of Technology. Her research focuses on immunization technology for the safety detection of agricultural products. She consulted the literature on design, helped solve problems in experiments, and provided technical guidance.

Corresponding author

Correspondence to Qingqing Yang.

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Wei, L., Zhang, H., Sun, X. et al. Aptasensor based on fluorescence resonance energy transfer for the determination of kanamycin. Eur Food Res Technol 248, 1563–1572 (2022). https://doi.org/10.1007/s00217-022-03985-1

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