Skip to main content
Log in

Influence of starter cultures and KCl on some biochemical, microbiological and sensory features of soppressata molisana, an Italian fermented sausage

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

The effect of a multiple strain starter (KSL: Kocuria varians, Staphylococcus xylosus and Lactobacillus sakei) on the quality of soppressata molisana was investigated during 50 days of ripening. When compared with control batches (C, traditional production without starter and KS, addition of K. varians and S. xylosus as starter), soppressata produced with KSL showed positive results in terms of proteolysis progression, reduction of biogenic amines (BA) and sensory features. The partial substitution of 50 % NaCl with 50 % KCl further improved the quality of soppressata molisana produced with KSL as starter. In fact, the K+ ions seemed to stimulate the growth of added bacteria, with a resulting additional lowering of the pH and BA formation, and higher proteolysis. Moreover, the addition of KCl did not alter the sensory profile of final products. The analysis of data collected during the fermentation and the drying phases, which followed one other, allowed the individuation of different proteolytic events. Specifically, the proteolysis of the myofibrillar fraction occurred more slowly than that of the sarcoplasmic one. Also, the analysis of fractionated muscle proteins allowed to appreciate that sarcoplasmic proteins were hydrolyzed by both endogenous and microbial enzymes, whereas myofibrillar proteins showed a higher resistance to microbial enzymes, and were primarily hydrolyzed by endogenous ones. In conclusion the combined use of KSL and KCl as partial substitute of NaCl, allowed the production of soppressata molisana having an excellent quality, and features consistent with the guidelines of WHO on the sodium and potassium intake.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Lücke FK (2000) Utilization of microbes to process and preserve meat. Meat Sci 56:105–115

    Article  Google Scholar 

  2. Coppola R, Iorizzo M, Giagnacovo B, Sorrentino A, Sorrentino E, Corino C, Grazia L (1995) La soppressata molisana: caratteristiche microbiologiche e tecnologiche. Ind Aliment 34:851–854

    Google Scholar 

  3. Coppola R, Iorizzo M, Saotta R, Sorrentino E, Grazia L (1997) Characterization of micrococci and staphylococci isolated from soppressata molisana, a Southern Italy fermented sausage. Food Microbiol 14:47–53

    Article  Google Scholar 

  4. Coppola R, Giagnacovo B, Iorizzo M, Grazia L (1998) Characterization of lactobacilli involved in the ripening of soppressata molisana, a typical southern Italy fermented sausage. Food Microbiol 15:347–353

    Article  Google Scholar 

  5. Aro-Aro JM, Nyam-Osor P, Tsuji K, Shimada K, Fukushima M, Sekikawa M (2010) The effect of starter cultures on proteolytic changes and amino acid content in fermented sausages. Food Chem 119(1):279–285

    Article  CAS  Google Scholar 

  6. Freiding S, Gutsche KA, Ehrmann MA, Vogel RF (2011) Genetic screening of Lactobacillus sakei and Lactobacillus curvatus strains for their peptidolytic system and amino acid metabolism, and comparison of their volatilomes in a model system. Syst Appl Microbiol 34(5):311–320

    Article  CAS  Google Scholar 

  7. Blom H, Hagen BF, Pedersen BO, Holck AL, Axelsson L, Naes H (1996) Accelerated production of dry fermented sausage. Meat Sci 43:S229–S242

    Article  Google Scholar 

  8. Leroy F, Verluyten J, De Vuyst L (2006) Functional meat starter cultures for improved sausage fermentation. Int J Food Microbiol 106:270–285

    Article  Google Scholar 

  9. Suzzi G, Gardini F (2003) Biogenic amines in dry fermented sausages: a review. Int J Food Microbiol 88:41–54

    Article  CAS  Google Scholar 

  10. Trani A, Gambacorta G, Loizzo P, Alviti G, Schena A, Faccia M, Aquilanti L, Santarelli S, Di Luccia A (2010) Biochemical traits of Ciauscolo, a spreadable typical Italian dry-cured sausage. J Food Sci 75:C514–C524

    Article  CAS  Google Scholar 

  11. Basso AL, Picariello G, Coppola R, Tremonte P, Spagna Musso S, Di Luccia A (2004) Proteolytic activity of Lactobacillus sakei, Lactobacillus farciminis and Lactobacillus plantarum on sarcoplasmic proteins of pork lean. J Food Biochem 28:195–212

    Article  CAS  Google Scholar 

  12. Picariello G, De Martino A, Mamone G, Ferranti P, Addeo F, Faccia M, Spagna Musso S, Luccia Di (2006) A proteomic study of muscle sarcoplasmic proteins using AUT-PAGE/SDS-PAGE as two-dimensional gel electrophoresis. J Chromatog 833:101–108

    CAS  Google Scholar 

  13. Nicastro A, La Gatta B, Picariello G, De Marzo D, Lamacchia C, Di Luccia A (2013) Proteolysis in fermented and not fermented dry cured salami. Prog Nutr 4:280–283

    Google Scholar 

  14. Armenteros M, Aristoy MC, Barat JM, Toldrá F (2009) Biochemical changes in dry-cured loins salted with partial replacements of NaCl by KCl. Food Chem 117:627–633

    Article  CAS  Google Scholar 

  15. Doyle ME, Glass KA (2010) Sodium reduction and its effect on food safety, food quality, and human health. Compr Rev Food Sci F 9(1):44–56

    Article  CAS  Google Scholar 

  16. Ruusunen M, Puolanne E (2005) Reducing sodium intake from meat products. Meat Sci 70(3):531–541

    Article  CAS  Google Scholar 

  17. Jiménez-Colmenero F, Carballo J, Cofrades S (2001) Healthier meat and meat products: their role as functional foods. Meat Sci 59:5–13

    Article  Google Scholar 

  18. WHO (2012) Guideline: sodium intake for adults and children. World Health Organization, Geneva

    Google Scholar 

  19. Aliño M, Grau R, Baigts D, Barat J (2009) Influence of sodium replacement on the salting kinetics of pork loin. J Food Eng 95(4):551–557

    Article  Google Scholar 

  20. Zanardi E, Ghidini S, Conter M, Ianieri A (2010) Mineral composition of Italian salami and effect of NaCl partial replacement on compositional, physico-chemical and sensory parameters. Meat Sci 86(3):742–747

    Article  CAS  Google Scholar 

  21. Lorenzo JM, Cittadini A, Bermúdez R, Munekata PE, Domínguez R (2015) Influence of partial replacement of NaCl with KCl, CaCl2 and MgCl2 on proteolysis, lipolysis and sensory properties during the manufacture of dry-cured lacòn. Food Control 55:90–96

    Article  CAS  Google Scholar 

  22. WHO (2012) Guideline: potassium intake for adults and children. World Health Organization, Geneva

    Google Scholar 

  23. Aljuraiban GS, Chan Q, Brown IJ, Griep LMO, Daviglus ML, Stamler J, Van Horn L, Holmes E, Elliott P, Frost GS (2012) 182 association of number of daily eating episodes with blood pressure and nutrient intake: intermap study. J Hypertension 30:e54–e55

    Article  Google Scholar 

  24. Gelabert J, Gou P, Guerrero L, Arnau J (2003) Effect of sodium chloride replacement on some characteristics of fermented sausages. Meat Sci 65:833–839

    Article  CAS  Google Scholar 

  25. Donfrancesco C, Ippolito R, Lo Noce C, Palmieri L, Iacone R, Russo O et al (2012) Excess dietary sodium and inadequate potassium intake in Italy: results of the MINISAL study. Nutr Metab Cardiovasc Dis. doi:10.1016/j.numecd.2012.04.004

    Google Scholar 

  26. Campanozzi A, Avallone S, Barbato A, Iacone R, Russo O, De Filippo G, D’Angelo G, Pensabene L, Malamisura B, Cecere G, Micillo M, Francavilla R, Tetro A et al (2015) High sodium and low potassium intake among Italian Children: relationship with age, body mass and blood pressure. PLoS One 10(4):e0121183

    Article  Google Scholar 

  27. Di Maria S, Basso AL, Santoro E, Grazia L, Coppola R (2002) Monitoring of Staphylococcus xylosus DSM 20266 added as starter during fermentation and ripening of soppressata molisana, a typical Italian sausage. J Appl Microbiol 92:158–164

    Article  Google Scholar 

  28. Tremonte P, Succi M, Di Renzo T, Reale A, Sorrentino E, Coppola R (2007) Interactions between strains of Staphylococcus xylosus and Kocuria varians isolated from fermented meats. J Appl Microbiol 103:743–751

    Article  CAS  Google Scholar 

  29. Sorrentino E, Tremonte P, Capobianco F, Succi M, Reale A, Di Renzo T, Coppola R (2007) Rapporti di interazione tra microrganismi di interesse tecnologico isolati da soppressata molisana. Ind Aliment 46:633–636

    Google Scholar 

  30. Tremonte P, Reale A, Di Renzo T, Tipaldi L, Di Luccia A, Coppola R, Sorrentino E E, Succi M (2010) Interactions between Lactobacillus sakei and CNC (Staphylococcus xylosus and Kocuria varians) and their influence on proteolytic activity. Lett Appl Microbiol 51:586–594

    Article  CAS  Google Scholar 

  31. Nazzaro F, Di Luccia A, Tremonte P, Grazia L, Sorrentino E, Maurelli L, Coppola R (2004) Evaluation of proteolytic activity of staphylococci in soppressata molisana, a typical Southern Italy fermented sausage. Ann Microbiol 54:269–281

    CAS  Google Scholar 

  32. Tremonte P, Tipaldi L, Succi M, Pannella G, Falasca L, Capilongo V, Coppola R, Sorrentino E (2014) Raw milk from vending machine: effects of boiling, microwave treatment and refrigeration on microbiological quality. J Dairy Sci 97:3314–3320

    Article  CAS  Google Scholar 

  33. Coppola R, Succi M, Sorrentino E, Iorizzo M, Grazia L (2003) Survey of lactic acid bacteria during the ripening of Caciocavallo cheese produced in Molise. Lait 83:211–222

    Article  CAS  Google Scholar 

  34. Reale A, Di Renzo T, Succi M, Tremonte P, Coppola R, Sorrentino E (2011) Identification of lactobacilli isolated in traditional ripe wheat sourdoughs by using molecular methods. World J Microbiol Biotechnol 27:237–244

    Article  Google Scholar 

  35. Di Luccia A, Picariello G, Cacace G, Scaloni A, Faccia M, Liuzzi V, Alviti G, Spagna Musso S (2005) Proteomic analysis of water soluble and myofibrillar protein changes occurring in dry-cured hams. Meat Sci 69:479–491

    Article  Google Scholar 

  36. Vinci G, Antonelli ML (2002) Biogenic amines: quality index of freshness in red and white meat. Food Control 13:519–524

    Article  CAS  Google Scholar 

  37. Meilgaard M, Civille GV, Carr BT (1999) Sensory evaluation techniques, 3rd edn. CRC PRESS, Boca Raton

    Book  Google Scholar 

  38. Berlitz HD, Grosh W, Schieberle P (2009) Food chemistry, 4th revised and extended edition. Springer, GmbH, Berlin, Heidelberg

  39. Kato H, Rhue MR, Nishimura T (1989) Role of free amino acids and peptides in food taste. In: Teranishi R, Buttery RG, Shahidi F (eds) Flavor chemistry. American Chemical Society, Washington

    Google Scholar 

  40. Ibañez C, Quintanilla L, Cid C, Astiasaran I, Belle J (1996) Dry fermented sausages elaborated with Lactobacillus plantarum-Staphylococcus carnosus Part I: effect of partial replacement of NaCl with KCl on the stability and the nitrosation process. Meat Sci 44:227–234

    Article  Google Scholar 

  41. Zanardi E, Ghidini S, Conter M, Ianieri A (2010) Mineral composition of Italian salami and effect of NaCl partial replacement on compositional, physico-chemical and sensory parameters. Meat Sci 86:742–747

    Article  CAS  Google Scholar 

  42. Bover-Cid S, Izquierdo-Pulido M, Vidal-Carou MC (1999) Effect of proteolytic starter cultures of Staphylococcus spp. on biogenic amine formation during the ripening of dry fermented sausages. Int J Food Microbiol 46:95–104

    Article  CAS  Google Scholar 

  43. Fadda S, Anglade P, Baraige F, Zagorec M, Talon R, Vignolo G, Champomier-Vergès MC (2010) Adaptive response of Lactobacillus sakei 23 K during growth in the presence of meat extracts: a proteomic approach. Int J Food Microbiol 142:36–43

    Article  Google Scholar 

  44. Spaziani M, Del Torre M, Stecchini ML (2009) Changes of physicochemical, microbiological, and textural properties during ripening of Italian low-acid sausages. Proteolysis, sensory and volatile profiles. Meat Sci 81:77–85

    Article  CAS  Google Scholar 

  45. Gou P, Guerrero L, Gelabert J, Arnau J (1996) Potassium chloride, potassium lactate and glycine as sodium chloride substitutes in fermented sausages and in dry-cured pork loin. Meat Sci 42:37–48

    Article  CAS  Google Scholar 

  46. Ohmizo C, Yata M, Katsu T (2004) Bacterial cytoplasmic membrane permeability assay using ion-selective electrodes. J Microbiol Methods 59:173–179

    Article  CAS  Google Scholar 

  47. Flores M, Toldrá F (2011) Microbial enzymatic activities for improved fermented meats. Review. Trends Food Sci Technol 22:81–90

    Article  CAS  Google Scholar 

  48. Kunji ER, Mierau I, Hagting A, Poolman B, Konings WN (1996) The proteolytic systems of lactic acid bacteria. Antonie Van Leeuwenhoek 70:187–221

    Article  CAS  Google Scholar 

  49. Fadda S, Sanz Y, Vignolo G, Aristoy MC, Oliver G, Toldrá F (1999) Characterization of muscle sarcoplasmic and myofibrillar protein hydrolysis caused by Lactobacillus plantarum. Appl Environ Microbiol 65:3540–3546

    CAS  Google Scholar 

  50. Xie C, Wang H, Nie X, Chen L, Deng S, Xu X (2015) Reduction of biogenic amine concentration in fermented sausage by selected starter cultures. CyTA J Food. doi:10.1080/19476337.2015.1005027

    Google Scholar 

  51. Latorre-Moratalla ML, Bover-Cid S, Bosch-Fusté J, Veciana-Nogués MT, Vidal-Carou MC (2014) Amino acid availability as an influential factor on the biogenic amine formation in dry fermented sausages. Food Control 36:76–81

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Elena Sorrentino.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics Requirements

No human participants and animals were involved.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Di Luccia, A., Tremonte, P., Trani, A. et al. Influence of starter cultures and KCl on some biochemical, microbiological and sensory features of soppressata molisana, an Italian fermented sausage. Eur Food Res Technol 242, 855–867 (2016). https://doi.org/10.1007/s00217-015-2591-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-015-2591-y

Keywords

Navigation