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Identification of antioxidant peptides from protein hydrolysates of scallop (Patinopecten yessoensis) female gonads

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Abstract

Scallop (Patinopecten yessoensis) female gonad hydrolysates (SFGHs) were obtained by using neutrase. The resulting hydrolysates possessed DPPH radical scavenging activity, ferrous ion-chelating ability, and reducing power with IC50 or AC0.5 values of 9.44, 0.94, and 5.88 mg/mL, respectively. SFGHs contained nearly 80 % of fractions with molecular weight around 250–5000 Da, and antioxidant-related amino acid residues in SFGHs reached to more than 30 %. Six fractions were separated from SFGHs on a Sephadex G-25 column, and one of the fractions with the highest DPPH radical scavenging activity was further analyzed by ESI-MS/MS, and a tetrapeptide His-Met-Ser-Tyr (536 Da) and a pentapeptide Pro-Glu-Ala-Ser-Tyr (565 Da) were identified. Both peptides showed hydroxyl radical scavenging activities with IC50 values of 3.6 and 16.8 mM, respectively, by electron spin resonance method. These results imply that peptides derived from scallop female gonads are potent antioxidants and may be utilized as functional ingredients in food systems.

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Abbreviations

SFG:

Scallop (Patinopecten yessoensis) female gonad

SFGHs:

Scallop (Patinopecten yessoensis) female gonad hydrolysates

DH:

Degree of hydrolysis

MW:

Molecular weight

ESR:

Electron spin resonance

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

DMPO:

5,5-dimethyl-1-pyrroline-N-oxide

BHA:

Butylated hydroxyanisole

Vc:

Vitamin C

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Acknowledgments

This work was financially supported by Public Science and Technology Research Funds Projects of Ocean (No. 201505029), the National Key Technology Research and Development Program of China during the 12th Five-Year Plan (No. 2014BAD04B09), Natural Science Fund of Liaoning Province (No. 2014026017), and Science and Technology Plan Projects of Liaoning Province (No. 2014205001).

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    Authors and Affiliations

    1. School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, People’s Republic of China

      Hai-Tao Wu, Shi-Guang Sun, Xiu-Hong Duan, Yi Li, Yu-Ting Yang, Jia-Run Han & Bei-Wei Zhu

    2. Bio-resources Key Laboratory of Shaanxi Province, School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, People’s Republic of China

      Wen-Gang Jin & Xin-Sheng Li

    3. National Engineering Research Center of Seafood, Dalian, 116034, People’s Republic of China

      Hai-Tao Wu & Bei-Wei Zhu

    4. National R&D Branch Center for Shellfish Processing, Dalian, 116034, Liaoning, People’s Republic of China

      Hai-Tao Wu & Bei-Wei Zhu

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    Hai-Tao Wu and Wen-Gang Jin have contributed equally to this work.

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    Wu, HT., Jin, WG., Sun, SG. et al. Identification of antioxidant peptides from protein hydrolysates of scallop (Patinopecten yessoensis) female gonads. Eur Food Res Technol 242, 713–722 (2016). https://doi.org/10.1007/s00217-015-2579-7

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