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Detection and quantification of melatonin and serotonin in eight Sweet Cherry cultivars (Prunus avium L.)

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Abstract

Melatonin functions as a free radical scavenger and controls the regulation of the sleep–wake cycle in mammals, while serotonin is the main intermediate in melatonin biosynthesis. In this paper, melatonin and serotonin have been detected and quantified for the first time in eight different sweet cherry cultivars using high performance liquid chromatography with mass spectrometry detection. The limits of detection of the proposed analytical method were 4.3 ng/mL for melatonin and 3.0 ng/mL for serotonin. An inverse relation between the contents of melatonin and serotonin was observed in the studied sweet cherry cultivars. The highest melatonin amounts were found in ‘Burlat’ sweet cherries (22.4 ± 1.3 ng/100 g of fresh fruit), while the highest serotonin contents were found in the cultivar ‘Ambrunés’ (37.6 ± 1.4 ng/100 g of fresh fruit). The results presented in this research allow us to know the amount of melatonin and serotonin bring to the diet.

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Acknowledgments

This research has been supported in part by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), research project RTA-2007-00096-C03-00, Junta de Extremadura and European Social Funds (PDT 08A008) and Convenio Universidad de Extremadura-Agrupación de Cooperativas Valle del Jerte (Re:067/07). D. González-Gómez and M.F. Fernández-León thank the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) for his research contract and her scholarship.

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Authors and Affiliations

  1. Technological Institute of Food and Agriculture of Extremadura (INTAEX), Ctra. San Vicente S/N, 06071, Badajoz, Spain

    D. González-Gómez, M. Lozano & M. F. Fernández-León

  2. Agricultural Engineering School, University of Extremadura, 06071, Badajoz, Spain

    M. C. Ayuso & M. J. Bernalte

  3. Science Faculty, University of Extremadura, 06071, Badajoz, Spain

    A. B. Rodríguez

Authors
  1. D. González-Gómez
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  2. M. Lozano
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  3. M. F. Fernández-León
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  4. M. C. Ayuso
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  5. M. J. Bernalte
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  6. A. B. Rodríguez
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Corresponding author

Correspondence to D. González-Gómez.

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González-Gómez, D., Lozano, M., Fernández-León, M.F. et al. Detection and quantification of melatonin and serotonin in eight Sweet Cherry cultivars (Prunus avium L.). Eur Food Res Technol 229, 223–229 (2009). https://doi.org/10.1007/s00217-009-1042-z

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  • DOI: https://doi.org/10.1007/s00217-009-1042-z

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