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Surface-enhanced Raman probe for rapid nanoextraction and detection of erythropoietin in urine

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Abstract

We present a surface-enhanced Raman probe (SERS) platform for the determination of a prohibited substance, recombinant erythropoietin (rEPO), in urine matrix, using nanoparticles as substrate. Rod-shaped gold nanoparticles (GNR) were modified with a Raman label and an antibody as SERS probe. We developed two SERS-based immunoassays for detection and quantification of rEPO in urine. In the first assay, rEPO was determined by a sandwich assay with gold surfaces and GNR. In the second assay, rEPO was extracted by using core shell-structured magnetic iron oxide gold nanoparticles, and again sandwich assay was performed by using GNR. We also demonstrated the ability of the proposed method to discriminate rEPO and urinary erythropoietin (uEPO). A good linear correlation was obtained between logarithms of rEPO concentrations in urine and Raman intensities within the range of 10−1–103 pg mL−1 rEPO concentrations. Detection limits which are smaller than 0.1 pg mL−1 levels were achieved owing to the high extractive performance of the nanoextraction techniques.

Schematic represantation of surface-enhanced Raman probe for rapid nanoextraction and detection of erythropoietin

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Acknowledgments

The authors are grateful for the financial supports provided by Gazi University BAP; Project Number: 46/2010-02 and 02/2011-14.

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Authors and Affiliations

  1. Turkish Doping Control Center, Hacettepe University, Sihhiye, 06100, Ankara, Turkey

    Yesim Somay Selbes & Arif Ahmet Basaran

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100, Ankara, Turkey

    Mehmet Gokhan Caglayan

  3. Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey

    Merve Eryilmaz & Ugur Tamer

  4. Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Ismail Hakki Boyaci

  5. Department of Nanotechnology and Nanomedicine, Institute of Science, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Necdet Saglam

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  1. Yesim Somay Selbes
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  3. Merve Eryilmaz
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Correspondence to Ugur Tamer.

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Selbes, Y.S., Caglayan, M.G., Eryilmaz, M. et al. Surface-enhanced Raman probe for rapid nanoextraction and detection of erythropoietin in urine. Anal Bioanal Chem 408, 8447–8456 (2016). https://doi.org/10.1007/s00216-016-9966-1

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  • DOI: https://doi.org/10.1007/s00216-016-9966-1

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