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Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

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Abstract

We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer–Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.

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Acknowledgments

This work was supported by the Department of Science and Technology, New Delhi, India. We thank Prof. P. Ramamurthy, Director, National Centre for Ultrafast Processes, University of Madras for lifetime measurements.

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Authors and Affiliations

  1. Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India

    E. Babu, S. Singaravadivel, G. Gnana kumar & S. Rajagopal

  2. Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India

    P. Manojkumar & S. Krishnasamy

Authors
  1. E. Babu
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  2. S. Singaravadivel
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  3. P. Manojkumar
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  4. S. Krishnasamy
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  5. G. Gnana kumar
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  6. S. Rajagopal
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Correspondence to S. Rajagopal.

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Babu, E., Singaravadivel, S., Manojkumar, P. et al. Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe. Anal Bioanal Chem 405, 6891–6895 (2013). https://doi.org/10.1007/s00216-013-7118-4

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  • DOI: https://doi.org/10.1007/s00216-013-7118-4

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