Abstract
Pichavaram mangrove forest was established as a wetland of International Importance by Article 2.1 in April 2022 by the Ministry of Environment, Forest and Climate Change, India. Even though it is a conserved site, xenobiotic agrochemical leaching on the forest land during monsoon is inevitable. These threaten the microbial diversity in the environment. Xenobiotic degradation is achieved using bacterial consortia already acclimatised to this environment. This study aims to identify the indigenous microbial consortia able to degrade xenobiotic compounds such as fluorobenzoate, furfural, and steroids. Pichavaram mangrove metagenomic dataset was obtained by shotgun sequencing of soil DNA and processed using the automated tool SqueezeMeta. Further, the DIAMOND database provided the taxonomical classification of the microbes in each contig. With reference to the KEGG database, the selected xenobiotic degradation pathways were confirmed in the dataset. Of 1,253,029 total contigs, 1332, 72 and 1262 were involved in fluorobenzoate, furfural and steroid degradation, respectively. This study identified that microbial consortia comprising Marinobacter, Methyloceanibacter and Vibrio natriegens/Gramella sp. can degrade fluorobenzoate. While Afipia, Nitrosopumilus sp., and Phototrophicus methaneseepsis favour the degradation of furfural compound. The steroid degradation pathway possessed a plethora of bacteria belonging to the phylum Proteobacteria.
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Data availability
The data that support the findings of this study are openly available in NCBI SRA database at https://www.ncbi.nlm.nih.gov/sra, reference number PRJNA1043081.
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Acknowledgements
The authors would like to thank SRM Insitute of Science and Technology for supporting the research. We are grateful for the continuous support and encouragement of our Department of Biotechnology SRMIST colleagues. Also, we thank the SRM-HPCC for supporting software and platforms to analyse the metagenomic data.
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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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LMS & PK— conceptualization, data curation, formal analysis, investigation, methodology, supervision, software, validation, visualization, writing original draft, review and editing. IV & BB — data curation and formal analysis.
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Communicated by Yusuf Akhter.
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Kannan, P., Verma, I., Banerjee, B. et al. Unveiling bacterial consortium for xenobiotic biodegradation from Pichavaram mangrove forest soil: a metagenomic approach. Arch Microbiol 206, 27 (2024). https://doi.org/10.1007/s00203-023-03765-9
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DOI: https://doi.org/10.1007/s00203-023-03765-9