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Halomonas xiaochaidanensis sp. nov., isolated from a salt lake sediment

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Abstract

A short-rod-shaped moderately halophilic bacterium, designated CUG 00002T, was isolated from the sediment of Xiaochaidan salt lake in Qinghai Province, China by using R2A medium. The cells were Gram-staining negative, aerobic, forming creamy and circular colonies with diameters of 2–3 mm on R2A agar when incubated at 30 °C for 3 days. 16S rRNA gene-based phylogenetic analysis indicated that strain CUG 00002T belonged to the genus Halomonas in the class Gammaproteobacteria, showing highest sequence similarity of 97.1 and 96.7 % to Halomonas mongoliensis Z-7009T (=DSM 17332=VKM B2353) and Halomonas shengliensis SL014B-85T (=CGMCC 1.6444T=LMG 23897T), respectively. The predominant isoprenoid quinone was ubiquinone-9 (Q9), and the major fatty acids were C16:0, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c) and summed feature 8 (comprising C18:1 ω7c or C18:1 ω6c). The genomic DNA G+C content of strain CUG 00002T was 61.8 mol%. The above characteristics were consistent with the placement of the organism in the genus Halomonas. The level of DNA–DNA relatedness between CUG 00002T and its most closely related strain H. mongoliensis Z-7009T was 41.0 ± 1.6 %. Based on the results of phenotypic, phylogenetic and biochemical analyses, strain CUG 00002T represents a novel species of the genus Halomonas, for which the name Halomonas xiaochaidanensis sp. nov. is proposed. The type strain is CUG 00002T (=CCTCC AB 2014152T=KCTC 42685T).

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Acknowledgments

We are grateful to Dr. Rüdiger Pukall (DSMZ, Germany) and Dr. Yuguang Zhou_(CGMCC, China) for their kind providing reference type strains. This research was supported by the National Natural Science Foundation of China (Grant No. 41422208), China Postdoctoral Science Foundation (2015M582298), State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences (No. GBL11201), and the Fundamental Research Funds for National University, China University of Geosciences (Wuhan). W-J Li was also supported by the Hundred Talents Program of Chinese Academy of Sciences and Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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Authors and Affiliations

  1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Wen Liu, Guojing Zhang, Jian Yang & Hongchen Jiang

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Wendong Xian, Lingling Yang & Wen-Jun Li

  3. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Min Xiao & Wen-Jun Li

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  1. Wen Liu
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  2. Guojing Zhang
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Correspondence to Hongchen Jiang or Wen-Jun Li.

Additional information

Communicated by Erko Stackebrandt.

The GenBank accession number for the 16S rRNA gene sequence of strain CUG 00002T is KU221020.

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Liu, W., Zhang, G., Xian, W. et al. Halomonas xiaochaidanensis sp. nov., isolated from a salt lake sediment. Arch Microbiol 198, 761–766 (2016). https://doi.org/10.1007/s00203-016-1235-3

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