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Teriparatide improves microarchitectural characteristics of peri-implant bone in orchiectomized rats

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Abstract

Summary

This study evaluated the peri-implant bone repair in orchiectomized rats receiving intermittently PTH 1-34. The treatment returned the bone quality and quantity of the animals to normal in the computerized microtomography, laser confocal microscopy, and histological analysis. The PTH 1-34 promoted marked bone formation with increased volume, improved quality, and greater bone turnover.

Introduction

Osteoporosis can be a problem in implant osseointegration. So this study aimed to evaluate the quantity and quality of peri-implant bone repair in orchiectomized Wistar rats receiving intermittently administered PTH 1-34.

Methods

Animals (n = 24) were divided into 3 groups: healthy control (SHAM), orchiectomized (ORQ), and orchiectomized and treated with 0.5 μg/kg/day PTH 1-34 (TERI), and each received an implant in the right and left tibial metaphysis, which was allowed to repair for 60 days. The resultant bone formation was evaluated through computerized microtomography (micro-CT) to compare the percent bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number and separation (Tb.N, Tb.Sp), and bone implant contact (BIC) through the intersection surface (i.S) between groups. Laser confocal microscopy was used to evaluate fluorochrome areas for mineral apposition rate (MAR) and neoformed bone area (NBA). In addition, histological evaluation of calcified tissues with Stevenel blue and alizarin red staining was performed.

Results

Treatment with PTH 1-34 returned the bone quality and quantity of the osteoporotic animal to normal, as the TERI group presented statistically significant higher values for BV/TV, Tb.Th, and BIC parameters compared with ORQ (p < 0.05), but when compared with SHAM (p > 0.05), no statistical difference was noted. In addition, in the bone turnover analysis (MAR, NBA) for TERI, the highest results are presented, followed by SHAM, and then ORQ (TERI × ORQ: p < 0.05).

Conclusions

Intermittent treatment with PTH 1-34 on orchiectomized animals promoted marked bone formation with increased volume, improved quality, and greater bone turnover in the peri-implant space, returning the bone quality and quantity to the present standard in healthy animals.

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Acknowledgments

The company Neodent is acknowledged for supplying the implants to make this work possible.

Funding

The authors would like to thank the São Paulo Research Foundation (FAPESP) for funding this work (process number: 2015/04649-7 and 2015/14688-0), CAPES (UNESP/CAPES/Print Project), the Department of Diagnosis and Surgery, as well as Prof. Dr. Elcio Marcantonio Júnior and laboratory technician Ana Cláudia Gregolin Costa Miranda, for facilitating the processing of the bone in the EXAKT system.

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Authors and Affiliations

  1. Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State University, Rua José Bonifácio, 1193, Vila Mendonça, Aracatuba, SP, 16015-050, Brazil

    P. H. S. Gomes-Ferreira & F. R. de Souza Batista

  2. Department of Basic Sciences, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil

    D. de Oliveira, P. B. Frigério & R. Okamoto

  3. Department of Materials Science and Engineering, McMaster University, Hamilton, ON, Canada

    K. Grandfield

  4. Research Productivity Scholarship (Process: 306389/2017-7), Aracatuba, SP, Brazil

    R. Okamoto

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  1. P. H. S. Gomes-Ferreira
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  3. P. B. Frigério
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  4. F. R. de Souza Batista
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Correspondence to P. H. S. Gomes-Ferreira.

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This work was approved by the Institutional Animal Ethics Committee of the Araçatuba Dental School (CEUA) with the registration number (CEUA: 2015-00238) and followed the ARRIVE guidelines for its preparation.

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Gomes-Ferreira, P.H.S., de Oliveira, D., Frigério, P.B. et al. Teriparatide improves microarchitectural characteristics of peri-implant bone in orchiectomized rats. Osteoporos Int 31, 1807–1815 (2020). https://doi.org/10.1007/s00198-020-05431-y

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