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Adsorption of Selenium and Lanthanum by Agrobacterium-mediated Hairy Roots of Hybanthus Enneaspermus (L.) F. Muell: A Greener Phytoremediation Strategy

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Abstract

In this study, phytoremediation potential of toxic metals like selenium (Se) and lanthanum (La) by transformed hairy roots was investigated and compared with plantlets under in-vitro conditions. Agrobacterium rhizogenes A4RS could induce hairy roots with higher biomass in 5–7 days of infection on in-vitro leaves of Hybanthus enneaspermus. The ICP-OES data indicated that the hairy roots were able to accumulate both selenium and lanthanum efficiently compared to plantlets. The hairy roots and plantlets show optimum absorption at 50 ppm under both individual and combined metal supply. The metal accumulation performances increased by 13.6% (La) and 10.9% (Se) in hairy roots with combined metal supply (La and Se) compared to individual supply (La or Se) conditions. The Se accumulated more than La, but the La accumulation percentage was found to increase substantially under combined metal supply conditions, shows the suitability and potential of hairy roots for phytoremediation of La and Se.

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References

  • Bahmani H, Maroufi A, Majdi M, Fakheri BA (2021) Thymol production in hairy root culture of Sahendian savory (Satureja sahendica Bornm). Plant Biotechnol Rep 15:177–186

    Article  CAS  Google Scholar 

  • Behera PR, Jena RC, Das A, Thirunavoukkarasu M, Chand PK (2016) Genetic stability and coumarin content of transformed rhizoclones and regenerated plants of a multi-medicinal herb, Hybanthus enneaspermus (L.) F. Muell. Plant Growth Regul 80:103–114

    Article  CAS  Google Scholar 

  • Bohidar S, Pattanaik S, Thirunavoukkarasu M (2013) Improved furano coumarin production in Ruta graveolens L. regenerated via in vitro stem internode cultures. Plant Biotechnol Rep 7:399–405

    Article  Google Scholar 

  • Cui J, Zhang Z, Bai W, Zhang L, He X, Ma Y, Liu Y, Chai Z (2012) Effects of rare earth elements La and Yb on the morphological and functional development of zebrafish embryos. J Environ Sci (China) 24:209–213

    Article  CAS  Google Scholar 

  • d’Aquino L, de Pinto MC, Nardi L, Morgana M, Tommasi F (2009) Effect of some light rare earth elements on seed germination, seedling growth and antioxidant metabolism in Triticum durum. Chemosphere 75:900–905

    Article  Google Scholar 

  • Domokos-Szabolcsy É, Fári M, Márton L, Czakó M, Veres S, Elhawat N, Antal G, el-Ramady H, Zsíros O, Garab G, Alshaal T (2018) Selenate tolerance and selenium hyperaccumulation in the monocot giant reed (Arundo donax), a biomass crop plant with phytoremediation potential. Environ Sci Pollut Res 25(31):31368–31380

    Article  CAS  Google Scholar 

  • Dridi N, Brito P, Bouslimi H, Ferreira R, Martins-Dias S, Caçador I, Sleimi N (2022) Physiological and biochemical behaviours and antioxidant response of Helianthus annuus under lanthanum and cerium stress. Sustainability 14(7):4153

    Article  CAS  Google Scholar 

  • Fathi R, Mohebodini M, Chamani E (2019) High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Cichorium intybus L. via removing macronutrients. Ind Crop Prod 128:572–580

    Article  CAS  Google Scholar 

  • Garousi F, Veres S, Kovács B (2016) Comparison of selenium toxicity in sunflower and maize seedlings grown in hydroponic cultures. Bull Environ Contam Toxicol 97:709–713

    Article  CAS  Google Scholar 

  • Gujarathi NP, Haney BJ, Park HJ, Wickramasinghe SR, Linden JC (2005) Hairy roots of Helianthus annuus: a model system to study phytoremediation of tetracycline and oxytetracycline. Biotechnol Prog 21:775–780

    Article  CAS  Google Scholar 

  • Haygarth PM, Fowler D, Stürup S, Davison BM, Tones KC (1994) Determination of gaseous and particulate selenium over a rural grassland in the U.K. Atmos Environ 28:3655–3663

    Article  CAS  Google Scholar 

  • Hu Z, Richter H, Sparovek G, Schnug E (2004) Physiological and biochemical effects of rare earth elements on plants and their agricultural significance: a review. J Plant Nutr 27:183–220

    Article  CAS  Google Scholar 

  • Ippolito MP, Paciolla C, d’Aquino LM, Morgana Tommasi F (2007) Effect of rare earth elements on growth and antioxidant metabolism in Lemna minor L. Caryologia 60:125–128

    Article  Google Scholar 

  • Jan R, Khan MA, Asaf S, Lee IJ, Kim KM (2019) Metal resistant endophytic bacteria reduces cadmium, nickel toxicity, and enhances expression of metal stress related genes with improved growth of Oryza sativa, via regulating its antioxidant machinery and endogenous hormones. Plants 8:363

    Article  CAS  Google Scholar 

  • Jha P, Sen R, Jobby R, Sachar S, Bhatkalkar S, Desai N (2020) Biotransformation of xenobiotics by hairy roots. Phytochem 176:112421

    Article  CAS  Google Scholar 

  • Li Z, Tian Y, Wang B, Peng R, Xu J, Fu X, Han H, Wang L, Zhang W, Deng Y, Wang Y, Gong Z, Gao J, Yao Q (2022) Enhanced phytoremediation of selenium using genetically engineered rice plants. J Plant Physiol 271:153665

    Article  CAS  Google Scholar 

  • Lv Q, Liang X, Nong K et al (2021) Advances in research on the toxicological effects of selenium. Bull Environ Contam Toxicol 106:715–726

    Article  CAS  Google Scholar 

  • Manan FA, Chai TT, Abd Samad A (2015) Environmental pollution in Malaysia: are medicinal plants potential phytoremediation agents. Int J Sci Technol 9:288

    Google Scholar 

  • Mechora Å, Rižnik T, Urbanek Krajnc A, Ambrožič-Dolinšek J (2020) Response of Berula erecta to lead in combination with selenium. Bull Environ Contam Toxicol 105:51–61

    Article  CAS  Google Scholar 

  • Mottaki Z, Rezayian M, Niknam V, Ebrahimzadeh H, Mirmasoumi M (2019) Using hairy roots for production of secondary metabolites in Artemisia. Plant Biotechnol Rep 13:263–271

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nedelkoska TV, Doran PM (2000) Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens. Biotechnol Bioeng 67:607–615

    Article  CAS  Google Scholar 

  • Pagano G, Aliberti F, Guida M, Oral R, Siciliano A, Trifuoggi M, Tommasi F (2015) Rare earth elements in human and animal health: state of art and research priorities. Environ Res 142:215–220

    Article  CAS  Google Scholar 

  • Palmer RJ, John L, Butenhoff Stevens JB (1987) Cytotoxicity of the rare earth metals cerium, lanthanum, and neodymium in vitro: comparisons with cadmium in a pulmonary macrophage primary culture system. Environ Res 43:142–156

    Article  CAS  Google Scholar 

  • Pandey N, Rai KK, Rai SK, Pandey-Rai S (2021) Heterologous expression of cyanobacterial PCS confers augmented arsenic and cadmium stress tolerance and higher artemisinin in Artemisia annua hairy roots. Plant Biotechnol Rep 15(3):317–334

    Article  CAS  Google Scholar 

  • Panichev A (2015) Rare earth elements: review of medical and biological properties and their abundance in the rock materials and mineralized spring waters in the context of animal and human geophagia reasons evaluation. Achiev Life Sci 9:95–103

    Google Scholar 

  • Rim KT, Koo KH, Park JS (2013) Toxicological evaluations of rare earths and their health impacts to workers: a literature review. Saf Health Work 4:12–26

    Article  CAS  Google Scholar 

  • Sirhindi G, Mir MA, Abd-Allah EF, Ahmad P, Gücel S (2016) Jasmonic acid modulates the physio-biochemical attributes, antioxidant enzyme activity, and gene expression in glycine max under nickel toxicity. Front Plant Sci 7:591

    Article  Google Scholar 

  • Sivanandhan G, Arunachalam C, Vasudevan V, Kapildev G, Sulaiman AA, Selvaraj N, Ganapathi A, Lim YP (2016) Factors affecting Agrobacterium-mediated transformation in Hybanthus enneaspermus (L.) F. Muell Plant Biotechnol Rep 10:49–60

    Article  Google Scholar 

  • Suresh B, Sherkhane PD, Kale S, Eapen S, Ravishankar GA (2005) Uptake and degradation of DDT by hairy root cultures of Cichorium intybus and Brassica juncea. Chemosphere 61:1288–1292

    Article  CAS  Google Scholar 

  • Vinterhalter B, Savic J, Platisa J, Raspor M, Ninkovic S, Mitic N (2008) Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldstet Kit. Plant Cell Tiss Organ Cult 94:299–303

    Article  CAS  Google Scholar 

  • Yan A, Wang Y, Tan SN, Yusof MLM, Ghosh S, Chen Z (2020) Phytoremediation: a promising approach for revegetation of heavy metal-polluted land. Front Plant Sci 11:359

    Article  Google Scholar 

  • Yang B-B, Yang C, Shao Z-Y, Wang H, Zan S-T, Zhu M, Zhou S-B, Yang R-Y (2019) Selenium (Se) does not reduce cadmium (cd) uptake and translocation in rice (Oryza sativa L.) in naturally occurred Se-rich paddy fields with a high geological background of cd.Bull Environ Contam Toxicol:1–6

  • Zhang CH, Li QQ, Zhang MX, Zhang N, Li MH (2013) Effects of rare earth elements on growth and metabolism of medicinal plants. Acta Pharm Sin B 3:20–24

    Article  Google Scholar 

  • Zhao C, Shi X, Xie S, Liu W, He E, Tang Y, Qiu R (2019) Ecological risk assessment of neodymium and yttrium on rare earth element mine sites in Ganzhou, China. Bull Environ Contam Toxicol 103:565–570

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to the Director, CSIR-NEERI for their support to carry out this research work at CSIR-NEERI (Chennai Zonal Laboratory). MN acknowledges the support from Department of Biotechnology (DBT), Govt. of India, New Delhi for the Ramalingaswami Re-Entry fellowship (No. BT/RLF/Re-entry/43/2018).

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Authors and Affiliations

  1. Chennai Zonal Laboratory, National Environmental Engineering Research Institute, 600113, Chennai, India

    Roshnara Mohanty & Manikkannan Thirunavoukkarasu

  2. Post-Graduate and Research Department of Botany, Pachaiyappa’s College, 600030, Chennai, India

    Roshnara Mohanty & Thangavel Sekar

  3. Amity Institute of Biotechnology, Amity University Uttar Pradesh, 201313, Noida, India

    Manoranjan Nayak

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  1. Roshnara Mohanty
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Correspondence to Manoranjan Nayak or Manikkannan Thirunavoukkarasu.

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Mohanty, R., Nayak, M., Sekar, T. et al. Adsorption of Selenium and Lanthanum by Agrobacterium-mediated Hairy Roots of Hybanthus Enneaspermus (L.) F. Muell: A Greener Phytoremediation Strategy. Bull Environ Contam Toxicol 110, 54 (2023). https://doi.org/10.1007/s00128-023-03694-9

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