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An intersubspecific genetic map of Lens

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Abstract

A Lens map was developed based on the segregational analysis of five kinds of molecular and morphological genetic markers in 113 F2 plants obtained from a single hybrid of Lens culinaris ssp. culinaris × L. c. ssp. orientalis. A total of 200 markers were used on the F2 population, including 71 RAPDs, 39 ISSRs, 83 AFLPs, two SSRs and five morphological loci. The AFLP technique generated more polymorphic markers than any of the others, although AFLP markers also showed the highest proportion (29.1%) of distorted segregation. At a LOD score of 3.0, 161 markers were grouped into ten linkage groups covering 2,172.4 cM, with an average distance between markers of 15.87 cM. There were six large groups with 12 or more markers each, and four small groups with two or three markers each. Thirty-nine markers were unlinked. A tendency for markers to cluster in the central regions of large linkage groups was observed. Likewise, clusters of AFLP, ISSR or RAPD markers were also observed in some linkage groups, although RAPD markers were more evenly spaced along the linkage groups. In addition, two SSR, three RAPD and one ISSR markers segregated as codominant. ISSR markers are valuable tools for Lens genetic mapping and they have a high potential in the generation of saturated Lens maps.

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Acknowledgements

This research was supported by the grants AGF99-0192 and AGL2002-0023 from the Spanish Dirección General de Investigación, and by personal grants to Y Duran (FPI from the Dirección General de Investigación) and R. Fratini from the Universidad de León.

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  1. Área de Genética, Facultad Ciencia Biológicas y Ambientales, Universidad de León, 24071, León, Spain

    Y. Durán, R. Fratini, P. García & M. Pérez de la Vega

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  1. Y. Durán
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  2. R. Fratini
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  3. P. García
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  4. M. Pérez de la Vega
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Correspondence to M. Pérez de la Vega.

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Communicated by H.C. Becker

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Durán, Y., Fratini, R., García, P. et al. An intersubspecific genetic map of Lens . Theor Appl Genet 108, 1265–1273 (2004). https://doi.org/10.1007/s00122-003-1542-3

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