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Simultaneous saccharification and fermentation of lignocellulose

Process evaluation

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Abstract

Simultaneous saccharification and fermentation (SSF) processes for producing ethanol from lignocellulose are capable of improved hydrolysis rates, yields, and product concentrations compared to separate hydrolysis and fermentation (SHF) systems, because the continuous removal of the sugars by the yeasts reduces the end-product inhibition of the enzyme complex. Recent experiments using Genencor 150L cellulase and mixed yeast cultures have produced yields and concentrations of ethanol from cellulose of 80% and 4.5%, respectively. The mixed culture was employed because B.clausenii has the ability to ferment cellobiose (further reducing end-product inhibition), while the brewing yeastS. cerevisiae provides a robust ability to ferment the monomeric sugars. These experimental results are combined with a process model to evaluate the economics of the process and to investigate the effect of alternative processes, conditions, and organisms.

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Authors and Affiliations

  1. Solar Energy Research Institute, 1617 Cole Blvd., 80401, Golden, CO

    John D. Wright, Charles E. Wyman & Karel Grohmann

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  1. John D. Wright
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  2. Charles E. Wyman
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  3. Karel Grohmann
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Wright, J.D., Wyman, C.E. & Grohmann, K. Simultaneous saccharification and fermentation of lignocellulose. Appl Biochem Biotechnol 18, 75–90 (1988). https://doi.org/10.1007/BF02930818

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  • DOI: https://doi.org/10.1007/BF02930818

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