Abstract
The intracellular enzyme activities ofChlorophyllum molybditis (Mayerex. Fr.) Massee andCortinarius melliolens Fries were determined at different stages of sporophore maturity. In both mushroom species, total amylase, α-amylase, proteinase, lipase, peroxidase, catalase and polyphenol oxidase activities were increased with sporophore maturity. In contrast, glucose-6-phosphatase activity was higher in the young sporophore than in very young and mature ones. All the enzymes assayed except cellulase and β-amylase showed greater activity in the pilei than in the stipes.C. molybditis showed greater total amylase, α-amylase, cellulase, proteinase, catalase and glucose-6-phosphatase activities thanC. melliolens.
Similar content being viewed by others
References
Denison, A.D., Koehn, D.R.: Cellulase activity ofPoronia oedipus.—Mycologia69: 592–603, 1977.
Fasidi, I.O., Kadiri, M.: Effect of maturity on the nutritive value of two Nigerian mushrooms,Tricholoma lobayensis Heim andCortinarius melliolens Fries.—Nigerian J. Bot.3: 41–47, 1990.
Feinstein, N.R.: Perborate as substrate in a new assay of catalase.—J. biol. Chem.180: 1197–1202, 1949.
Gruen, H.E., Wong, M.W.: Distribution of cellular amino acids protein and total organic nitrogen during fruitbody development inFlammulina velutipes. 1. Growth on sawdust medium.—Can. J. Bot.60: 1330–1341, 1982.
Hammond, W.B.J.: Variations in enzyme activity during periodic fruiting ofAgaricus bisporus.—New Phytol.89: 419–428, 1981.
Hammond, W.B.J., Nichols, R.: Changes in respiration and soluble carbohydrates during the postharvest storage of mushroom (Agaricus bisporus).—J. Sci. Food Agr.26: 835–842, 1975.
Jablonsky, L.: Changes in biochemical and physiological activities of substrates colonized by fungiPleurotus ostreatus, Lentinus edodes andAgrocybe aegirita.—Mushroom Sci.11: 659–673, 1981.
Kadiri, M., Fasidi, I.O.: Studies on enzyme activities ofPleurotus tuber-regium (Fries) Singer andTricholoma lobayensis Heim at various fruitbody stages.—Nährung34: 695–699, 1990a.
Kadiri, M., Fasidi, I.O.: Variations in chemical composition ofChlorophyllum molybditis (Mayerex Fr.) Massee andPleurotus tuber-regium (Fries) during fruitbody development.—Nigerian J. Sci.24: 86–88, 1990b.
Keilin, D., Hartree, F.E.: Purification of horse radish peroxidase and comparison of its properties with those of catalase and methaemoglobin.—Biochem. J.49: 88–104, 1951.
Lowry, O.H., Rosebrough, H.N., Farr, L.A., Randall, J.R.: Protein measurement with the Folin phenol reagent.—J. biol. Chem.193: 265–275, 1951.
McDonald, E.C., Chen, L.L.: Lowry modification of the Folin reagent for determination of proteinase activity.—Anal. Biochem.10: 175–177, 1965.
Moore, D., Elhiti, Y.M.M., Butler, D.R.: Morphogenesis of the carpophore ofCoprinus cinereus.— New Phytol.83: 695–722, 1979.
Norkrans, B.: Studies of β-glucoside and cellulose splitting enzymes fromPolyporus annosus Fr..—Physiol. Plant.10: 198–214, 1957.
Paranjpe, S.M., Chen, K.P.: Morphogenesis ofAgaricus bisporus: Changes in proteins and enzyme activity.—Mycologia71: 469–478, 1979.
Russell, J.L.A.: The estimation of phosporus.—Biochem. J.34: 858–865, 1940.
Singh, N., Kunene, S.I.: Cellulase decomposition by four isolates ofPyricularia oryzae.—Mycologia72: 182–190, 1980.
Swain, R.R., Dekker, E.E.: Seed germination studies 1. Purification and properties of an α-amylase from the cotyledons of germinating peas.—Biochim. biophys. Acta122: 77–86, 1966.
Swanson, M.A.: Determination of glucose-6-phosphatase activity.—In: Colowick, S.P., Kaplan, N. (ed.): Methods in Enzymology. Vol. II. Pp. 541–542. Academic Press, New York 1955.
Wang, C.W.: Cellulolytic enzymes ofVolvariella esculenta.—In: Chang, S.T., Quimio, T.N. (ed.): Tropical Mushrooms: Biological Nature and Cultivation Methods. Pp. 167–186. Chinese University Press, Hong Kong 1982.
Wilson, M.A.: Amylase synthesis and stability in crested wheat grass seeds at low water potentials.— Plant Physiol.48: 541–546, 1971.
Yamaguchi, M.H., Hwung, M.M., Campbell, D.J.: Latento-diphenol oxidase in mushroom (Agaricus bisporus).—Can. J. Biochem.48: 198–202, 1970.
Yong, F.M., Wood, B.J.B.: Biochemical changes in experimental soya sauce (Koji).—J. Food Technol.12: 163–175, 1977.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kadiri, M., Fasidi, I.O. Enzyme activities ofChlorophyllum molybditis andCortinarius melliolens at different sporophore stages. Biol Plant 36, 607–611 (1994). https://doi.org/10.1007/BF02921189
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02921189