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Bioconversion of fumaric acid to succinic acid by recombinantE. coli

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Abstract

Succinic acid was produced efficiently from fumaric acid by a recombinantE. coli strain DH5αt/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. L-Malic acid became the major fermentation product under these conditions. Provision of glucose (5-30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.

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Authors and Affiliations

  1. Laboratory of Renewable Resources Engineering, 1295 Potter Engineering Center, Purdue University, 47907, West Lafayette, IN

    Xiaohai Wang, C. S. Gong & George T. Tsao

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  1. Xiaohai Wang
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  2. C. S. Gong
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  3. George T. Tsao
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Wang, X., Gong, C.S. & Tsao, G.T. Bioconversion of fumaric acid to succinic acid by recombinantE. coli . Appl Biochem Biotechnol 70, 919–928 (1998). https://doi.org/10.1007/BF02920202

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  • DOI: https://doi.org/10.1007/BF02920202

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