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Isolation of polysaccharide-free DNA from plants

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Abstract

A quick procedure for the isolation of polysaccharide-free DNA from different plant species and cell suspension or callus cultures is described. The originality of the method lies in the use of a mixture of glycoside hydrolases that leads, after phenol and chloroform extraction, to the isolation of pure DNA without any polysaccharide contamination. The highly purified DNA can be used for nucleotide analysis by HPLC, RFLP analysis and PCR amplification.

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Authors
  1. Benoît Rether
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  2. Geneviève Delmas
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  3. Abdelkrim Laouedj
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Rether, B., Delmas, G. & Laouedj, A. Isolation of polysaccharide-free DNA from plants. Plant Mol Biol Rep 11, 333–337 (1993). https://doi.org/10.1007/BF02905335

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  • DOI: https://doi.org/10.1007/BF02905335

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