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Isolation of DNA from plants high in polyphenolics

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Abstract

DNA suitable for digestion with restriction endonucleases has been isolated fromTheobroma cacao, a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. The method relies on concentrating nuclei away from most of the cytoplasmic components and inhibiting the formation of oxidized polyphenolic compounds and their interaction with the DNA fraction in the remaining steps.

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Authors and Affiliations

  1. ACRI Cocoa Molecular Biology Lab, The Intercollege Program of Genetics, Pennsylvania State University, 16802, University Park, PA, USA

    Jennifer A. Couch & Paul J. Fritz

  2. The Department of Food Science, Pennsylvania State University, 16802, University Park, PA, USA

    Jennifer A. Couch & Paul J. Fritz

Authors
  1. Jennifer A. Couch
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  2. Paul J. Fritz
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Couch, J.A., Fritz, P.J. Isolation of DNA from plants high in polyphenolics. Plant Mol Biol Rep 8, 8–12 (1990). https://doi.org/10.1007/BF02668875

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  • DOI: https://doi.org/10.1007/BF02668875

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