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Fine structure and assemblyin vitro of nuclear lamina in plant cells

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Abstract

The fine structure of the nuclear lamina (NL) in sperm cells ofGinkgo biloba was visualised using high resolution low-voltage scanning electron microscopy (LVSEM). It was shown that the nuclear lamina was composed of 10 nm filaments which formed a fine network. Lamins were purified from cultured carrot suspension cells and assembledin vitro. Long 8–12 nm diameter filaments were seen and sometimes subfilaments could be distinguished. Western blot of filament preparations showed that these contained the 66 and 84 ku lamins. These data demonstrate that plant lamins are capable of assembling into filamentsin vitro.

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Author notes

  1. Jian Wang

    Present address: Department of Biochemistry, Max Planck Institute for Biophysical Chemistry, PO Box 2841, D-37018, Goettingen, Germany

Authors and Affiliations

  1. College of Life Sciences, Peking University, 100871, Beijing, China

    Jian Wang, Cheng Yang & Zhonghe Zhai

  2. Integrated Microscopy Resource, University of Wisconsin, Madison, USA

    Ris Hans

Authors
  1. Jian Wang
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  2. Cheng Yang
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  3. Ris Hans
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  4. Zhonghe Zhai
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Project supported by the National Natural Science Foundation of China (Grant No. 3500073).

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Wang, J., Yang, C., Hans, R. et al. Fine structure and assemblyin vitro of nuclear lamina in plant cells. Sci. China Ser. C.-Life Sci. 41, 71–79 (1998). https://doi.org/10.1007/BF02882708

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  • DOI: https://doi.org/10.1007/BF02882708

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