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Cloning and sequence analysis of the invertase geneINV1 from the yeastPichia anomala

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Abstract

A genomic library from the yeastPichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant ofSaccharomyces cerevisiae. The cloned gene,INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also withKluyveromyces marxianus inulinase, a yeast β-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5′ and 3′ non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.

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Authors and Affiliations

  1. Departamento de Microbiologia y Biologia Celular, Facultad de Farmacia, Universidad de La Laguna, E-38071, La Laguna, Tenerife, Spain

    José A. Pérez, Javier Rodriguez, Luis Rodriguez & Teresa Ruiz

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  1. José A. Pérez
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  2. Javier Rodriguez
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  3. Luis Rodriguez
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  4. Teresa Ruiz
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Communicated by C.P. Hollenberg

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Pérez, J.A., Rodriguez, J., Rodriguez, L. et al. Cloning and sequence analysis of the invertase geneINV1 from the yeastPichia anomala . Curr Genet 29, 234–240 (1996). https://doi.org/10.1007/BF02221553

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  • DOI: https://doi.org/10.1007/BF02221553

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