Abstract
Growth, substrates and end-product formation of the maltose and citrate co-metabolization byLactobacillus brevis subsp.lindneri CB1 citrate-negative strain were initially studied in synthetic medium. Compared to maltose (19 g/l) fermentation, the co-metabolization of maltose (10 g/l) plus citrate (9 g/l) caused faster cell growth, increased the concentrations of lactic acid and especially of acetic acid (from 0.7 g/l to 2.9 g/l), produced succinic acid (0.5 g/l) and reduced ethanol synthesis. Highest activities of acetate kinase, the same of lactate dehydrogenase and a reduced alcohol dehydrogenase activity were detected in cytoplasmic extracts of cells growing on maltose plus citrate. The breakdown of citrate depended upon the continuous presence of maltose in the growth medium. Upon depletion of citrate, the cells continued through the normal maltose fermentation, having a diauxic metabolic curve as shown by impedance measurements. Concentrations of citrate from 3 g/l to 15 g/l led to increases of acetic acid from 1.25 g/l to 5.55 g/l. Since maltose was naturally present during sourdough fermentation, the addition of 9 g citrate per kg wheat dough enabled the co-metabolization of maltose and citrate byL. brevis subsp.lindneri CB1. Compared with traditional sourdough fermentation, faster cell growth, a higher acetic acid concentration and a reduced quotient of fermentation were obtained by co-metabolism.
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Kennes C, Veiga MC, Dubourguier HC, Touzel JP, Albagnac G, Naveau H, Nyns EJ (1981) Appl Environ Microbiol 57: 1046–1051
Axelsson LT (1993) In: Salminen S, von Wright A (eds) Lactic acid bacteria. Dekker, New York, pp 1–63
da Cunha MV, Foster MA (1992) J Bacteriol 174: 1013–1019
Cogan TM (1987) J Appl Bacteriol 63: 551–558
Schmitt P, Diviès C, Merlot C (1990) Biotechnol Lett 12: 127–130
Schmitt P, Bernet N, Zarzell Y, Diviès C (1994) Milchwissenschaft 49: 183–185
Talarico TL, Axelsson LT, Novotny J, Fiuzat M, Dobrogosz WJ (1990) Appl Environ Microbiol 56: 943–948
Gobbetti M, Corsetti A, Rossi J (1995) Appl Microbiol Biotechnol 42: 939–944
Lütgens M, Gottschalk G (1980) J Gen Microbiol 119: 63–70
Gobbetti M, Corsetti A, Rossi J (1994) Appl Microbiol Biotechnol 41: 456–460
Gobbetti M, Corsetti A, Rossi J (1994) World J Microbiol Biotechnol 10: 275–279
Gobbetti M, Simonetti MS, Rossi J, Cossignani L, Corsetti A, Damiani P (1994) J Food Sci 59: 881–884
Gobbetti M, Corsetti A, Rossi J (1994) Microbiol Alim Nutr 12: 9–15
Gobbetti M, Corsetti A, Rossi J, La Rosa F, De Vincenzi S (1994) Ital J Food Sci 1: 85–94
Kline L, Sugihara TF (1971) Appl Microbiol 21: 459–465
Bergmeyer HU, Graßl M, Walter HS (1983) In: Bergemeyer HU (ed) Methods of enzymatic analysis, vol. 2. Weinheim, Deerfield Beach, Fla., pp 126–326
Smith AF (1983) In: Bergemeyer HU (ed) Methods of enzymatic analysis, vol. 3. Weinheim, Deerfield Beach, Fla., pp 166–171
Wahlefeld AW (1983) In: Bergemeyer HU (ed) Methods of enzymatic analysis, vol. 3. Weinheim, Deerfield Beach, Fla., pp 126–133
Walsh B, Cogan TM (1974) J Dairy Res 41: 25–30
Quaglia G (1984) In: Quaglia G (ed) Scienza e tecnologia della panificazione. Chiriotti, Pinerolo, Italy, pp 295–323
Okigbo LM, Oberg CJ, Richardson GH (1985) J Dairy Sci 68: 2521–2527
Drinan DF, Tobin S, Cogan TM (1976) Appl Environ Microbiol 31: 481–486
Radier F (1973) In: Carr JG, Cutting CV, Whiting GC (eds) Lactic acid bacteria in beverages and food. Academic Press, London, pp 17–35
Axelsson LT, Lindgren S (1987) J Appl Bacteriol 62: 433–438
Spicher G (1983) In: Rehm HJ, Reed G (eds) Biotechnology, vol. 5. Verlag Chemie, Weinheim, Fla., pp 1–80
Correra C (1994) Il Latte 9: 866–869
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Gobbetti, M., Corsetti, A. Co-metabolism of citrate and maltose byLactobacillus brevis subsp.lindneri CB1 citrate-negative strain: effect on growth, end-products and sourdough fermentation. Z Lebensm Unters Forch 203, 82–87 (1996). https://doi.org/10.1007/BF01267775
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DOI: https://doi.org/10.1007/BF01267775