Skip to main content
SpringerLink
Log in

Variation in neurovirulence of NWS influenza virus after repeated passages in different tissue culture systems

I. Derivation and neurotropic activity of NWS virus lines

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

The NWS virus was repeatedly passaged in four different tissue cultures: cercopithecus monkey kidney cells, rhesus monkey kidney cells, human diploid cells (strain LEP) and chick embryo fibroblasts. After 10 or 11 passages virus stocks were prepared in chick embryos and tested in white mice for neurovirulence. Only one of the four virus lines, denoted as NWS-R, retained the high neurotropic activity of the original NWS virus, while two other lines proved almost without neurotropic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Stuart-Harris, C. H.: Lancetii, 497 (1939).

    Google Scholar 

  2. Francis, T., Jr., andA. E. Moore: J. exp. Med.72, 717 (1940).

    Google Scholar 

  3. Wong, S. C., andE. D. Kilbourne: J. exp. Med.113, 95 (1961).

    PubMed  Google Scholar 

  4. Kilbourne, E. D., A. Sugiura, andS. C. Wong: Proc. Soc. exp. Biol. (N.Y.)116, 225 (1964).

    Google Scholar 

  5. Kutinová, L., V. Vonka, andD. Řezáčová: Acta virol.11, 372 (1967).

    PubMed  Google Scholar 

  6. Vonka, V.: Arch. ges. Virusforsch.15, 514 (1965).

    PubMed  Google Scholar 

  7. Michl, J.: Exp. Cell Res.23, 324 (1961).

    PubMed  Google Scholar 

  8. Michl, J.: Exp. Cell Res.26, 129 (1962).

    PubMed  Google Scholar 

  9. Org. mond. Santé Sér. Rapp. techn., 170 (1959).

Download references

Author information

Author notes

  1. Z. Janda

    Present address: Virus Department, Research Institute of Immunology, Prague, Czechoslovakia

Authors and Affiliations

  1. Virus Department, Research Institute of Immunology, Prague, Czechoslovakia

    V. Vonka

Authors
  1. Z. Janda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Vonka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Janda, Z., Vonka, V. Variation in neurovirulence of NWS influenza virus after repeated passages in different tissue culture systems. Archiv f Virusforschung 24, 192–196 (1968). https://doi.org/10.1007/BF01241291

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01241291

Keywords

Search

Navigation