Abstract
Lipids were extracted from bovine brain myelin using a mixture of hexane and isopropanol (3∶2). Myelin lipids were resolved, using Sep Pak chromatography, into four fractions: Fraction 1 contained neutral lipids, fraction 2, free fatty acids, fraction 3, ethanolamine phospholipids and fraction 4, choline phospholipids. Docosahexanoic (DHA) and arachidonic (AA) acids in these fractions were measured by RPHPLC. Fraction 2 was analyzed directly, the other three fractions were subjected to alkaline hydrolysis before analysis for DHA and AA. DHA and AA were not found in fraction 1. Both DHA and AA were found in fractions 2 and 3. Only AA was consistently found in fraction 4. These results were confirmed by GC.
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References
Esiri, M. M., and Reading M. C. 1987. Macrophage populations associated with multiple sclerosis plaques. Neuropath. Appl. Neurobiol. 13:451–465.
Kishimoto, Y., Radin, N. S., Tourtellotte, W. W., Parker, J. A., and Itabashi, H. H. 1967. Gangliosides and glycerophospholipids in multiple sclerosis white matter. Arch. Neurol. 16:44–54.
Gerstl, B., Tavaststjerna, M. G., Hayman, R. B., Eng, L. F., and Smith, J. K. 1965. Alterations in myelin fatty acids and plasmalogens in multiple sclerosis. Ann. N.Y. Acad. Sci. 122:405–415.
Trotter, J., and Smith, M. E. 1985. The role of phospholipases from inflammatory macrophages in demyelination. Neurochem. Res. 11:349–361.
Konat, G. W., and Wiggins, R. C. 1985. Effect of reactive oxygen species on myelin membrane proteins. J. Neurochem. 45:1113–1118.
Compston, A. 1988. Methyl prednisolone and multiple sclerosis. Arch. Neurol. 45:669–670.
Stefoski, D., Davis, F.A., and Schauf, C.L. 1985. Acute improvement in exacerbating multiple sclerosis produced by intravenous administration of mannitol. Ann. Neurol. 18:443–450.
Norton, W. T., and Poduslo, S. E. 1973. Myelination in rat brain: Method of myelin isolation. J. Neurochem. 21:749–757.
Bonting, S. L., and Caravaggio, L. L. 1963. Studies on sodiumpotassium-activated adenosinetriphosphatase. V. Correlation of enzyme activity with cation flux in six tissues. Arch. Biochem. Biophys. 101:37–46.
Prohask, J. R., Clark, D. A., and Wells, W. W. 1973. Improved rapidity and precision in the determination of brain 2′,3′-cyclic nucleotide 3′-phosphohydrolase. Anal. Biochem. 56:275–282.
Markwell, M. A. K., Haas, S. M., Bieber, L. L., and Tolbert, N. E. 1978. A modification of the Lowry protein procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87:206–210.
Norton, W. T., and Poduslo, S. E. 1973. Myelination in rat brain: Changes in myelin composition during brain maturation. J. Neurochem. 21:759–773.
Ansari, K. A., and Shoeman, D. 1988. Rapid separation of free fatty acids from neutral lipids in brain extracts. J. Chromatog. 439:453–458.
Sun, G. Y., and Horrocks, L. A. 1968. The fatty acid and aldehyde composition of the major phospholipids of mouse brain. Lipids 3:79–83.
Sun, G. Y., and Horrocks, L. A. 1971. The acyl and alk-l-enyl groups of the major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions. Lipids 12:1006–1012.
Sun, G. Y., and Sun, A. Y. 1972. Phospholipids and acyl groups of synaptosomal and myelin membranes isolated from the cerebral cortex of squirrel monkey (saimiri sciureus). Biochem. Biophys. Acta 280:306–315.
Blaker, W. D., and Moscatelli, E. A. 1978. The effect of hibernation on the lipids of brain myelin and microsomes in the syrian hamster. J. Neurochem. 31:1513–1518.
Bourre, J. M., Pascal, G., Durand, G., Masson, M., Dumont, O., and Piciotti, M. 1984. Alterations in the fatty acid composition of rat brain cells (Neurons, astrocytes and oligodendrocytes) and of subcellular fractions (myelin and synaptosomes) induced by a diet devoid of n-3 fatty acids. J. Neurochem. 43:342–348.
O'Brien, J. S., and Sampson, E. L. 1965. Fatty acid and fatty aldchyde composition of the major brain lipids in normal human gray matter, white matter, and myelin. J. Lipid Res. 6:545–551.
Fressinaud, C., Rigaud, M., and Vallat, J. M. 1986. Fatty acid composition of endoneurium and perineurium from adult rat sciatic nerve. J. Neurochem. 46:1549–1553.
Aveldano, M. I., VanRollins, M., and Horrocks, L. A. 1983. Separation and quantitation of free fatty acids and fatty acid methyl esters by reverse phase high pressure liquid chromatography. J. Lipid Res. 24:83–93.
Hartung, H-P., Schafer, B., Heininger, K., Stoll, G., and Toyka, C. V. 1988. The role of macrophages and eicosanoids in the pathogenesis of experimental allergic neuritis. Brain 111:1039–1059.
Dore-Duffy, P., Siok, C., and Donaldson, J. O. 1982. Prostaglandin E synthesis in patients with multiple sclerosis: correlation with disease activity. Neurology 32:164A.
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Ansari, K.A., Shoeman, D.W. Arachidonic and docosahexanoic acid content of bovine brain myelin: Implications for the pathogenesis of multiple sclerosis. Neurochem Res 15, 7–11 (1990). https://doi.org/10.1007/BF00969177
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DOI: https://doi.org/10.1007/BF00969177