Summary
HindIII digested DNA from various mutant strains of Saccharomyces cerevisiae probed with a 340 by nucleotide sequence in M13mp8 derived from a mouse liver cDNA clone p1581 showed strong hybridization to a 4.1 kb DNA fragment class. This was limited to the DNA of cells of alpha mating type but the fragments concerned apparently do not originate from chromosome III. The pattern of hybridization was modified in strains carrying the HO gene consistent with there being extra copies of the Bkm-homologous sequence in these cells. Northern analysis of RNA from cells synchronised in various stages of the mitotic and meiotic cell cycle probed with MI 3mp8/p1581 indicated related transcripts in meiotic cells.
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References
Aviv H, Leder P (1972) Proc Nat Acad Sci USA 69:1408–1412
Gupta NJ (1981) PhD thesis, Calcutta University
Hawthorne DC (1963) Proc Int Congr Genet (Abstr) 1:34–35
Hicks JB, Strathern JN, Herskowitz I (1977) In: Bukhari A, Shapiro J, Adhya S (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 457
Jensen R, Sprague GGF, Herskowitz I (1983) Proc Natl Acad Sci USA 80:3035–3038
Jinks-Robertson S, Petes TD (1985) Proc Natl Acad Sci USA 82:3350–3354
Jones KW, Singh L (1981a) In: Dover G, Flavell R (eds) Genome evolution, 20. Academic, London, pp 135–154
Kurtz S, Lindquist S (1984) Proc Natl Acad Sci USA 81:7323–7327
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Miller AM, MacKay VL, Nasmyth KA (1985) Nature 314:598–603
Nasmyth KA (1983) Nature 302:670–675
Nasmyth KA, Tatchell DB, Hall C, Astell C, Smith M (1981) Nature 289:244–250
Phillipsen P, Thomas M, Kramer A, Davis RW (1978) J Mol Biol 123:387–404
Silverman SJ, Fink GR (1984) Mol Cell Biol 4:1246–1251
Simchen G, Idar D, Kassir Y (1976) Mol Gen Genet 144:21–27
Singh L, Jones KW (1984) Nucleic Acid Res 12:5627–5638
Singh L, Purdom IF, Jones KW (1980) Chromosoma 79:137–157
Singh L, Purdom IF, Jones KW (1981) Cold Spring Harbor Symp Quant Biol 45:805–813
Singh L, Phillips C, Jones KW (1984) Cell 36:111–120
Slater ML (1973) J Bacteriol 113:263–270
Stern M, Jensen RE, Herskowitz I (1984) J Mol Biol 178:853–868
Strathern JN, Spatola E, McGill C, Hicks JB (1980) Proc Natl Acad Sci USA 77:2839–2843
Walker M-J (1985) PhD thesis, University of Edinburgh
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Gupta, N.J., Jones, K.W. A DNA sequence which shows genomic variation in a, alpha and HO strains of Saccharomyces cerevisiae . Curr Genet 12, 161–166 (1987). https://doi.org/10.1007/BF00436874
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DOI: https://doi.org/10.1007/BF00436874