Summary
Randomly selected clones from a Phytophthora infestans partial genomic library were characterized by hybridizing individual clones to Southern blots of total genomic DNA digested with the restriction enzyme EcoRI. Among 59 clones that were screened on seven different central-Mexican isolates, five revealed a unique banding pattern for each isolate tested. Two of these clones were tested further; the banding patterns produced by both were somatically stable when probed to DNA from 63 single-zoospore (asexual) progeny from five different “parent” isolates. For one probe, RG57, each band appeared to represent a unique genetic locus in three different crosses, and each locus segregated for the presence or absence of a band. No bands were found to be allelic, but two pairs of cosegregating loci were identified. Genetic analyses of the other probe (RG7) revealed many more pairs of cosegregating bands and some bands which were allelic. When these probes were hybridized to DNA from the other five species in Phytophthora group IV, probe RG57 hybridized strongly to DNA from P. colocasiae, P. phaseoli and P. mirabilis, but weakly or not at all to that of P. hibernalis and P. ilicis. Probe RG7 hybridized fairly strongly to DNA from all six species. Because the sequence recognized by probe RG57 appears to be evolutionarily conserved, and is dispersed, moderately repetitive and highly polymorphic, it could be very useful in additional studies on the genetics and population biology of P. infestans.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene Publishing Associates/Wiley Interscience, New York
Braithwaite KS, Manners JM (1989) Curr Genet 16: 473–475
Burke T, Bruford MW (1987) Nature 327: 149–152
Caten CE, Jinks JL (1968) Can J Bot 46: 329–348
Dallas JF (1988) Proc Natl Acad Sci USA 85: 6831–6835
Feinberg AP, Vogelstein B (1983) Anal Biochem 132: 6–13
Francis DM, Hulbert SH, Michelmore RW (1990) Exp Mycol 14: 299–309
Galindo J, Hohl HR (1985) Sydowia, Ann Mycol Ser II 38: 87–96
Garber RC, Yoder OC (1983) Anal Biochem 135: 416–422
Hamer JE, Givan S (1990) Mol Gen Genet 223: 487–495
Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG (1989) Proc Natl Acad Sci USA 86: 9981–9985
Hooker WJ (1981) Compendium of potato diseases. The American Phytopathological Society, St. Paul, Minnesota, USA
Houck MA, Clark JB, Peterson KR, Kidwell MG (1991) Science 253: 1125–1129
Jeffreys AJ, Morton DB (1987) Animal Genet 18: 1–15
Jeffreys AJ, Wilson V, Thein SL (1985a) Nature 314: 67–73
Jeffreys AJ, Wilson V, Thein SL (1985b) Nature 316: 76–79
Jeffreys AJ, Wilson V, Thein SL, Weatherall DJ, Ponder BAJ (1986) Am J Hum Genet 39: 11–24
Jeffreys AJ, Royle NJ, Wilson V, Wong Z (1988) Nature 332: 278–281
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fulimoto E, Hoff M, Kumlin E, White R (1987) Science 235: 1616–1622
Sansome E, Brazier CM (1973) Nature 241: 344–345
Scherer S, Stevens DA (1988) Proc Natl Acad Sci USA 85: 1452–1456
Shattock RC, Tooley PW, Fry WE (1986) Phytopathology 76: 410–413
Shaw DS (1991) Adv Plant Pathol 7: 131–170
Spielman LJ (1991) Isozymes and the population genetics of Phytophthora infestans. In: Lucas JA, Shattock RC, Shaw DS, Cooke LR (eds) Phytophthora.. Cambridge University Press, Cambridge, pp 231–241
Syvanen M (1987) J Mol Evol 26: 16–23
Waterhouse GM (1963) Mycological Papers, no. 92. Commonwealth Mycological Institute, Kew
Westneat DF (1990) Behav Ecol Sociobiol 27: 67–76
Wetton JH, Carter RE, Parkin DT Walters D (1987) Nature 327: 147–149
Author information
Authors and Affiliations
Additional information
Communicated by H. Bertrand
Rights and permissions
About this article
Cite this article
Goodwin, S.B., Drenth, A. & Fry, W.E. Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs from Phytophthora infestans . Curr Genet 22, 107–115 (1992). https://doi.org/10.1007/BF00351469
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00351469