Abstract
Incorporation of 3H-uridine into three chromosome regions 21D, 100AB, 7EF showing no puffs was studied by means of EM autoradiography. These regions show rather good coincidence between EM and Bridges' revised maps. The reduction of band number observed in the EM map was mainly at the expense of “doublet” bands. — Theoretical silver grain distributions were calculated on the basis of “universal curves” (Salpeter et al., 1969, J. Cell Biol. v. 41, 1–20) on condition that either bands or interbands are linear sources of radioactivity. From these curves the resolution of EM autoradiography was deduced to be sufficient with regard to the investigated region. — The results show that in addition to the puffs peaks of silver grains occur over the interbands and diffuse bands. The lowest incorporation level is observed over the dense bands. The possibility of utilizing the data obtained for the location of RNA-synthesising regions is discussed.
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Ananiev, E.V., Barsky, V.E.: Localization of RNA synthesis sites in the 1B-3C region of the Drosophila melanogaster X-chromosome. Chromosoma (Berl.) 65, 359–371 (1978)
Ashburner, M.: The puffing activities of the salivary gland chromosomes. In: Handbook of genetics (R.C. King, ed.), vol. 3, p. 793. New York-London: Plenum Press 1975
Atherton, D., Gall, J.: Salivary gland squashes for in situ nucleic acid hybridization studies. Dros. Inf. Serv. 49, 131–133 (1972)
Beermann, W.: Chromomeres and genes. In: Results and problems in cell differentiation (W. Beermann, ed.) vol. 4, 1–33. Berlin, Heidelberg, NewYork: Springer 1972
Beermann, W., Bahr, A.F.: The submicroscopic structure of the Balbiani ring. Exp. Cell Res. 6, 195–202 (1954)
Belyaeva, E.S., Zhimulev, I.F., Semeshin, V.F.: Cytogenetic analysis of 2B1-2 emdash 2B9-10 region of Drosophila melanogaster X-chromosome. Communication I. Cytological mapping of chromosome rearrangements and regularities of puffing in the region. Genetika (U.S.S.R.) (in press, 1979)
Belyaeva, E.S., Korochkina, L.S., Zhimulev, I.F., Nazarova, N.K.: Characteristics of puffs at female X-chromosomes of Drosophila melanogaster. Tsitologie (U.S.S.R.) 16, 440–446 (1974)
Berendes, H.D.: Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma (Berl.) 17, 35–77 (1965)
Clever, U., Romball, C.G.: RNA and protein synthesis in the cellular response to a hormone ecdysone. Proc. nat. Acad. Sci (Wash.) 56, 1470–1476 (1966)
Derksen, J.: The submicroscopic structure of synthetically active units in a puff of Drosophila hydei giant chromosomes. Chromosoma (Berl.) 50, 45–52 (1975)
Derksen, J., Berendes, H.D., Willart, E.: Production and release of a locus-specific ribonucleoprotein product in polytene nuclei of Drosophila hydei. J. Cell Biol. 59, 661–668 (1973)
Elgin, S.C.R., Silver, L.M., Wu, C.E.C.: The in situ distribution of Drosophila non-histone chromosomal proteins. In: The molecular biology of the mammalian genetic apparatus (P. Ts'o, ed.) pp. 127–141. Amsterdam: Elsevier North-Holland Biomedical Press 1977
Ephrussi, B., Beadle, G.B.: Technique of transplantation for Drosophila. Amer. Naturalist 70, 218–225 (1936)
Fujita, B., Takamoto, K.: Synthesis of messenger RNA on the polytene chromosomes of dipteran salivary glands. Nature (Lond.) 200, 494–495 (1963)
Gersh, E.S.: Sites of gene activity and of inactive genes in polytene chromosomes of Diptera. J. theor. Biol. 50, 413–428 (1975)
Jamrich, M., Greenleaf, A.L., Bautz, E.K.F.: Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 74, 2079–2083 (1977)
Kaufmann, T.C.: Characterization of 3 new alleles of the giant locus of Drosophila melanogaster. Genetics, 71, Suppl., 28–29 (1972)
Kerkis, A.Ju., Zhimulev, I.F., Belyaeva, E.S.: Method of electronmicroscopic autoradiography for studying structural and functional organization of particular regions of polytene chromosomes. Tsitologia (U.S.S.R.) 17, 1330–1331 (1975)
Kerkis, A. Ju., Zhimulev, I.F., Belyaeva, E.S.: EM autoradiographic study of 3H-uridine incorporation into D. melanogaster salivary gland chromosomes. Dros. Inf. Serv. 52, 14–17 (1977)
Khristolyubova, N.B., Kerkis, A.Ju.: The application of the “light” radioautography for electron microscopy investigations. Tsitologia (U.S.S.R.) 10, 1496–1499 (1968)
Kiknadze, I.I.: Analysis of puff activity in the course of the larval development of Diptera and experimental treatments. In: Cell differentiation and inductive mechanisms (G.V. Lopashov, ed.) pp. 78–99. Moscow: Nauka 1965
King, R.C.: Drosophila melanogaster: an introduction. In: Handbook of genetics (R.C. King, ed.), vol. 3, p. 625, New York, London: Plenum Press 1975
Lakhotia, S.C., Jacob, J.: EM autoradiographic studies on polytene nuclei of Drosophila melanogaster. 2. Organization and transcriptive activity of the chromocentre. Exp. Cell Res. 86, 253–263 (1974)
Lefevre, G.: A photographic representation and interpretation of the polytene chromosomes of Drosophila melanogaster. In: The genetics and biology of Drosophila (M Ashburner and E. Novitski, eds.) vol. 1a, pp. 32–66. London: Academic Press 1976
Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627, 1968
Pelling, C.: Ribonucleins:aure-Synthese der Riesenchromosomen.Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964)
Perov, N.A., Chentsov, Y.S.: Electron microscopic studies of the larval salivary gland chromosomes of Chironomus plumosus. Dokl. Akad. Nauk USSR 196, 1452–1456 (1971)
Perov, N.A., Kiknadze, I.I., Chentsov, Y.S.: Ultrastructural organization of the salivary gland polytene chromosomes of Chironomus thummi. Tsitologia (U.S.S.R.) 17, 390–397 (1975a)
Perov, N.A., Kiknadze, I.I., Chentsov, Y.S.: Specific ultrastructure of puffs in the region 4-E2b-e in Chironomus thummi. Dokl. Acad. Nauk USSR 223, 1465–1467 (1975b)
Salpeter, M.M., Bachmann, L., Salpeter, E.E.: Resolution in electron microscope radioautography. J. Cell Biol. 41, 1–20 (1969)
Saura, A.O.: Salivary gland chromosome 2L of Drosophila melanogaster, an electron microscopic mapping. Helsinki Chromosome Conference, Abstract book, p. 55. Helsinki 1977
Semeshin, V.F., Zhimulev, I.F., Belyaeva, E.S.: Cytogenetic study of 9E-10A region of Drosophila melanogaster X-chromosome. 1. Morphology of the region and mapping of the deficiencies affecting the band 10A1-2. Genetika (U.S.S.R.) (in press, 1979)
Skaer, R.J.: Interband transcription in Drosophila. J. Cell Sci. 26, 251–266 (1977)
Sorsa, M., Sorsa, V.: Electronmicroscopic studies on band regions in Drosophila salivary chromosomes. Ann. Acad. Sci. fenn., Ser. A, IV, Biol. 127, 1–9 (1968)
Vazquez-Nin, G., Bernhard, W.: Comparative ultrastructural study of perichromatin and Balbiani ring granules. J. Ultrastruct. Res. 36, 842–860 (1971)
Zhimulev, I.F.: Comparative study of the function of polytene chromosomes in laboratory stocks of Drosophila melanogaster the I(3)tl mutant (lethal tumorous larvae). 1. Analysis of puffing patterns in autosomes of the laboratory stock Batumi-L. Chromosoma (Berl.) 46, 59–76 (1974)
Zhimulev, I.F., Belyaeva, E.S.: 3H-uridine labelling patterns in Drosophila melanogaster salivary chromosomes. Genetica (U.S.S.R.) 10, 71–79 (1974)
Zhimulev, I.F., Belyaeva, E.S.: 3H-uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3L. Chromosoma (Berl.) 49, 219–231 (1975a)
Zhimulev, I.F., Belyaeva, E.S.: Proposals to the problem of structural and functional organization of polytene chromosomes. Theoret. Appl. Genet. 45, 335–340 (1975b)
Zhimulev, I.F., Lytchev, V.A.: RNA synthesis in the left arm of the III chromosome in Drosophila melanogaster during the last 5 hours of larval development. Ontogenez (U.S.S.R.) 3, 289–298 (1972)
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Semeshin, V.F., Zhimulev, I.F. & Belyaeva, E.S. Electron microscope autoradiographic study on transcriptional activity of Drosophila melanogaster polytene chromosomes. Chromosoma 73, 163–177 (1979). https://doi.org/10.1007/BF00331569
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DOI: https://doi.org/10.1007/BF00331569