Summary
In this report we describe the procedure of growing human lymphocytes with the demethylating agent S-adenosyl-l-homocysteine (SAH). After this treatment, which is not toxic for cell survival, both R-and G-banding were obtained by new experimental procedures: R-bands have been directly demonstrated with the GC-specific fluorochrome chromomycin A3 without the necessity of any AT-specific counterstaining agent; simultaneous G-banding and active nucleolar organizer regions have been obtained by silver impregnation of chromosomes and subsequent Giemsa staining. These results suggest a possible relationship between local differences in DNA methylation and the determination of the banded chromosome structure.
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References
Antequera F, McLeod D, Bird AP (1989) Specific protection of methylated CpGs in mammalian nuclei. Cell 58:507–509
Barbés C, Sánchez J, Yebra MJ, Robert-Geró M, Hardisson C (1990) Effects of sinefungin and S-adenosylhomocysteine on DNA and protein methyltransferases from Streptomyces and other bacteria. FEMS Microbiol Lett 69:239–244
Felsenfeld C, Nickol J, Behe M, McGhee J, Jackson D (1982) Methylation and chromatin structure. Cold Spring Harbor Symp Quant Biol 67:577–584
Fernández-Piqueras J, Rodriguez-Campos A, Sentís C, Rojo E (1982) Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure. Chromosoma 85:707–711
Holmquist GP (1989) Evolution of chromosome bands: molecular ecology of noncoding DNA. J Mol Evol 28:469–486
Keshet I, Lieman-Hurwitz J, Cedar H (1986) DNA methylation affects the formation of active chromatin. Cell 44:535–543
Manuelidis L (1990) A view of interphase chromosomes. Science 250:1533–1540
Schmid M, Haaf T, Grunert D (1984) 5-Azacytidine-induced undercondensations in human chromosomes. Hum Genet 67:257–263
Schweizer D (1981) Counterstain-enhanced chromosome banding. Hum Genet 57:1–14
Ueland PM (1982) Pharmacological and biochemical aspects of S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase. Pharmacol Rev 34:223–253
Yau ED, Coward JL (1990) Synthesis of complex 6'-alkynyl-6'-dethia nucleoside analogues of S-adenosylhomocysteine as potential inhibitors of methyltransferases. J Org Chem 55:3147–3158
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De Cabo, S.F., Sentís, C. & Fernández-Piqueras, J. Induction of G- and R-banding in human chromosomes by the demethylating agent S-adenosyl-l-homocysteine. Histochemistry 97, 499–501 (1992). https://doi.org/10.1007/BF00316070
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DOI: https://doi.org/10.1007/BF00316070