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A new gene encoding tRNAPro (GGG) is present in the chloroplast genome of black pine: a compilation of 32 tRNA genes from black pine chloroplasts

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Abstract

The chloroplast genome of black pine (Pinus thumbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNAPro (GGG) and tRNAArg (CCG); the former is newly identified while the latter is present in liverwort, Physcomitrella patens and Angiopteris lygodiifolia, chloroplast genomes. Moreover, a partial copy of the split tRNAGly (UCC) gene and full copies of tRNAHis (GUG), tRNAThr (GGU) and tRNASer (GCU) genes are present in the large single-copy region of the genome, suggesting extensive rearrangements of the chloroplast genome during evolutio. No tRNA genes whose tRNA products can recognize codons CUU/C (Leu) and GCU/C (Ala) have been found. We propose that the 32 tRNAs are sufficient to read all the 61 sense codons in the black pine system using the “two-out-of-three” and the “U:N wobble” mechanisms.

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Authors and Affiliations

  1. Sugiyama Jogakuen University, Chikusa, 464, Nagoya, Japan

    Junko Tsudzuki & Shiho Ito

  2. Aichi-Gakuin University, 470-01, Nisshin, Aichi, Japan

    Takahiko Tsudzuki

  3. Center for Gene Research, Nagoya University, 464-01, Nagoya, Japan

    Junko Tsudzuki, Shiho Ito, Tatsuya Wakasugi & Masahiro Sugiura

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  1. Junko Tsudzuki
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  2. Shiho Ito
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  3. Takahiko Tsudzuki
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  4. Tatsuya Wakasugi
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  5. Masahiro Sugiura
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Tsudzuki, J., Ito, S., Tsudzuki, T. et al. A new gene encoding tRNAPro (GGG) is present in the chloroplast genome of black pine: a compilation of 32 tRNA genes from black pine chloroplasts. Curr Genet 26, 153–158 (1994). https://doi.org/10.1007/BF00313804

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  • DOI: https://doi.org/10.1007/BF00313804

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