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Mutations in the structural gene for cytochrome c result in deficiency of both cytochromes aa 3 and c in Neurospora crassa

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Abstract

The cyt-12-12 mutant of Neurospora crassa is characterized by slow growth and a deficiency of spectrophotometrically-detectable cytochromes aa 3 and c. Using a sib-selection procedure we have isolated the cyt-12 + allele from a cosmid library of N. crassa genomic DNA. Characterization of the cyt-12 + allele reveals that it encodes the structural gene for cytochrome c. DNA sequence analysis of the cyt-12-12 allele revealed a mutation in the cytochrome c coding sequence that results in replacement of a glycine residue, which is invariant in the cytochrome c of other species, with an aspartic acid. Genetic analysis confirms that cyt-12-12 is allelic with the previously-characterized cyc-1-1 mutant, which was also shown to affect the single locus encoding cytochrome c in N. crassa. We suggest that the amount of functional cytochrome c present in mitochondria influences the level of cytochrome aa 3 .

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Authors and Affiliations

  1. Department of Genetics, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada

    Drell A. Bottorff, Sukran Parmaksizoglu, Edmond G. Lemire, John W. Coffin & Frank E. Nargang

  2. Department of Microbiology, Michigan State University, 48824-1011, East Lansing, MI, USA

    Helmut Bertrand

Authors
  1. Drell A. Bottorff
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  2. Sukran Parmaksizoglu
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  3. Edmond G. Lemire
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  4. John W. Coffin
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  5. Helmut Bertrand
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  6. Frank E. Nargang
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Communicated by K. Wolf

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Bottorff, D.A., Parmaksizoglu, S., Lemire, E.G. et al. Mutations in the structural gene for cytochrome c result in deficiency of both cytochromes aa 3 and c in Neurospora crassa . Curr Genet 26, 329–335 (1994). https://doi.org/10.1007/BF00310497

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  • DOI: https://doi.org/10.1007/BF00310497

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