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Demonstration of endocytosis vesicles by means of electron-microscopic histochemistry

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Summary

A phosphatase activity similar to that of the plasma membrane is demonstrated at the inside of the membrane, which delimits the lipid-containing lysosomes of chicken liver parenchymal cells. This is taken as a definite confirmation of their endocytotic origin.

It should be possible to demonstrate endocytosis vesicles in other cells in this way. It is, however, pointed out, that cells with a weak plasma membrane activity may require particular attention to certain steps in the technical procedure.

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Authors and Affiliations

  1. Department of Human Anatomy, University of Uppsala, Uppsala, Sweden

    Björn Sandström

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  1. Björn Sandström
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This study was supported in part by a grant from the Swedish Medical Research Council.

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Sandström, B. Demonstration of endocytosis vesicles by means of electron-microscopic histochemistry. Histochemie 26, 197–200 (1971). https://doi.org/10.1007/BF00305651

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  • DOI: https://doi.org/10.1007/BF00305651

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