Abstract
The chromosomal changes occurred in two independent cell lines (GM2 and GM3) of Drosophila melanogaster maintained in medium supplemented with serum and in serum-free medium were compared. In both culture conditions and in both lines a chromosomal evolution was revealed. Structural and numerical variations were analysed. The breaks giving rise to rearrangements were at heterochromatic level. Moreover, a tetraploidisation followed by loss of chromosomes or of portions of chromosomes recalls an analogous cycle observed in human cells.
Similar content being viewed by others
References
Adkisson, K.P., Perreault, W.J., Gay, H.: Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard. Chromosoma (Berl.) 34, 190–205 (1971)
Dolfini, S.: Karyotype polymorphism in a cell population of Drosophila melanogaster cultured in vitro. Chromosoma (Berl.) 33, 196–208 (1971)
Dolfini, S., Gottardi, A.: Changes of chromosome number in cells of Drosophila melanogaster cultured in vitro. Experientia (Basel) 22, 144 (1966)
Echalier, G., Ohanessian, A.: In vitro culture of Drosophila melanogaster embryonic cells. In Vitro 6, 162–172 (1970)
Ellison, J.R., Barr, H.J.: Difference in the quinacrine staining of the chromosomes of a pair of sibling species: Drosophila melanogaster and Drosophila simulans. Chromosoma (Berl.) 34, 424–435 (1971)
Faccio Dolfini, S.: Spontaneous chromosome rearrangements in an established cell line of Drosophila melanogaster, Chromosoma (Berl.) 47, 253–261 (1974)
Gatti, M., Tanzarella, C, Olivieri, G.: Analysis of the chromosome aberrations induced by X-rays in somatic cells of Drosophila melanogaster. Genetics 77, 701–719 (1974)
Halfer, C., Tiepolo, L., Barigozzi, C., Fraccaro, M.: Timing of the DNA replication of translocated Y chromosome sections in somatic cells of Drosophila melanogaster. Chromosoma (Berl.) 27, 395–408 (1969)
Horikawa, M., Fox, A.S.: Culture of embryonic cells of Drosophila melanogaster in vitro. Science 145, 1437–1439 (1964)
Kaufmann, B.P.: Somatic mitoses of Drosophila melanogaster. J. Morph. 56, 125–155 (1934)
Martin, G.M., Sprague, C.A.: Parasexual cycle in cultivated human somatic cells. Science 166, 761–763 (1969)
Mosna, G.: Medium for Drosophila cells in vitro without serum. Dros. Inf. Serv. 49, 60 (1972)
Mosna, G., Dolfini, S.: Morphological and chromosomal characterization of three new continuous cell lines of Drosophila melanogaster. Chromosoma (Berl.) 38, 1–9 (1972)
Terzi, M., Hawkins, T.S.C.: Chromosomal variation and the establishment of somatic cell lines in vitro. Nature (Lond.) 253, 361–362 (1975)
Vosa, C.G.: The discriminating fluorescence patterns of the chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 31, 446–451 (1970)
Zuffardi, O., Tiepolo, L., Dolfini, S., Barigozzi, C., Fraccaro, M.: Changes in the fluorescence patterns of translocated Y chromosome segments in Drosophila melanogaster. Chromosoma (Berl.) 34, 274–280 (1971)
Author information
Authors and Affiliations
Additional information
This work was supported by a grant of the Consiglio Nazionale delle Ricerche, Roma.
Rights and permissions
About this article
Cite this article
Dolfini, S.F. Karyotype evolution in cell lines of Drosophila melanogaster . Chromosoma 58, 73–86 (1976). https://doi.org/10.1007/BF00293442
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00293442