Summary
Transcripts of the Petunia mitochondrial cytoplasmic male sterility (CMS)-associated S-Pcf locus, which consists of three co-transcribed genes (pcf, NADH dehydrogenase subunit 3, and ribosomal protein S12), have been characterized in reproductive tissues of CMS and fertility restored (Rf) Petunia lines by nuclease protection experiments and by RNA blot hybridization. Three 5′ transcript termini have been previously described. Two 3′ transcript termini and an additional S-Pcf locus transcript have now been identified. The relative abundance of the three 5′ transcript termini is influenced by the presence of the nuclear Rf gene. A decrease in the abundance of the −121 5′ transcript terminus relative to the −266 and −522 termini is consistently seen in Petunia lines which are restored to fertility by the Rf gene, compared to CMS Petunia lines. An additional transcript with a 5' terminus within the urf-s region of pcf is much more abundant in immature bud and anther tissue than in leaf or suspension cells. The total abundance of pcf transcripts varies greatly between plants of different nuclear backgrounds which lack the nuclear Rf allele, indicating that other nuclear genes also influence expression of the S-Pcf locus.
Similar content being viewed by others
References
Bino RJ (1985a) Histological aspects of microsporogenesis in fertile, cytoplasmic male sterile and restored fertile Petunia hybrida. Theor Appl Genet 69:423–428
Bino RJ (1985b) Ultrastructural aspects of cytoplasmic male sterility in Petunia hybrida. Protoplasma 127:230–240
Boeshore ML, Hanson MR, Izhar S (1985) A variant mitochondrial DNA arrangement specific to Petunia stable sterile somatic hybrids. Plant Mol Biol 4:125–132
Feinberg A, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–11
Hanson MR, Conde MF (1989) Male sterility loci in plant mitochondrial genomes. Oxford Surveys Plant Mol Cell Biol 6:61–85
Hanson MR, Boeshore ML, McClean PE, O'Connell MA, Nivison HT (1986) The isolation of mitochondria and mitochondrial DNA. Methods Enzymol 118:437–453
Izhar S (1984) Male Sterility in Petunia. In: Sink KC (ed) Monographs on Theoretical and Applied Genetics, Petunia, vol 9. Springer-Verlag, New York, pp 76–91
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual, 1st edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
van Marrewijk GAM, Bino RJ, Suurs LCJM (1986) Characterization of cytoplasmic male sterility in Petunia hybrida. I. Localization, composition and activity of esterases. Euphytica 35:77–88
Nivison HT, Hanson MR (1989) Identification of a mitochondrial protein associated with cytoplasmic male sterility in Petunia. Plant Cell 1:1121–1130
Poutre CG, Fox TD (1987) PET111, a Saccharomyces cerevisiae nuclear gene required for translation of the mitochondrial mRNA encoding cytochrome c oxidase subunit II. Genetics 115:637–647
Pruitt KD, Hanson MR (1989) Cytochrome oxidase subunit lI sequences in Petunia mitochondria: two intros-containing genes and an intros-less pseudogene associated with cytoplasmic male sterility. Curr Genet 16:281–291
Pruitt KD, Hanson MR (1991) Splicing of the Petunia cytochrome oxidase subunit II intron. Curr Genet 19:191–197
Rasmussen J, Hanson MR (1989) A NAD dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility. Mol Gen Genet 215:332–336
Rothenberg M, Hanson MR (1988) A functional mitochondrial ATP synthase proteolipid gene produced by recombination of parental genes in a Petunia somatic hybrid. Genetics 118:155–161
Wu FS, Murry LE (1985) Changes in protein and amino acid content during anther development in fertile and cytoplasmic male sterile Petunia. Theor Appl Genet 71:68–73
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49
Young EG, Hanson MR, Dierks PM (1986) Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit. Nucleic Acids Res 14:7995–8006
Author information
Authors and Affiliations
Additional information
Communicated by R.G. Herrmann
Rights and permissions
About this article
Cite this article
Pruitt, K.D., Hanson, M.R. Transcription of the Petunia mitochondrial CMS-associated Pcf locus in male sterile and fertility-restored lines. Molec. Gen. Genet. 227, 348–355 (1991). https://doi.org/10.1007/BF00273922
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00273922