Summary
Radioactive penicillin G production from l-[1-14C]-valine (1.75 GBq · mmol-1) by native and by calcium alginate gel immobilized mycelium of Penicillium chrysogenum PQ-96 in a medium for antibiotic production as well as by vesicles isolated from the protoplasts of the same strain in a well-defined reaction mixture was investigated. Specific radioactivity of the penicillin G produced by the native vesicles was 1.45 GBq · mmol-1 and that of the antibiotic synthesized by the calcium alginate gel immobilized vesicles was 1.48 GBq · mmol-1. By comparison, the specific radioactivity of penicillin G produced by native mycelium was 0.42 GBq · mmol-1 and of that synthesized by the immobilized mycelium was 0.96 GBq · mmol-1. Production of radioactive penicillin G by native and immobilized vesicles in repeated use was also investigated. At the beginning of the production phase, the radioactive penicillin G synthesized by the immobilized vesicles was 25 nmol · mg protein-1 · h-1 and decreased after 8 days to a level of 11 nmol · mg protein-1 · h-1. The half-life of the immobilized vesicles was 7 days. The native vesicles showed a rapid decrease in radioactive antibiotic production. In comparison, the penicillin G production in a repeated use of immobilized vesicles decreased during 40 days from 140 nmol · mg protein-1 · h-1 to 60 nmol · mg protein-1 · h-1. The half-life of the immobilized vesicles was 35 days. The native vesicles showed after 4 days a lack of activity of penicillin G production. The stability of immobilized mycelium or vesicles in the process of radioactive penicillin G production is discussed.
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References
Dentice di Accadia F, Carta-De Angeli L (1961) Metodi di analisi quantitative applicati albrodi di fermentazione. Instituto Superiore di Sanita, Roma
Grove DC, Randall WA (1955) Assay methods of antibiotics. A laboratory manual. Medical Encyclopedia, Inc, New York
Kierstan M, Bucke C (1977) The immobilization of microbial cells, subcellular organelles and enzymes in calcium alginate gels. Biotechnol Bioeng 19:387–397
Kluender H, Bradley CH, Sih CJ, Fawcett P, Abraham EP (1973) Synthesis and incorporation of (2S, 3S)-[4-13C]-valine into β-lactam antibiotics. J Am Chem Soc 95:6149–6150
Kuryłowicz W, Kurz atkowski W, Woźnicka W, Połowniak-Pracka H, Paszkiewicz A (1979a) The ultrastructure of Penicillium chrysogenum in the course of benzyl-penicillin biosynthesis. Zbl Bakt II Abt 134:706–720
Kuryłowicz W, Kurz atkowski W, Woźnicka W, Połowniak-Pracka H, Paszkiewicz A (1979b) The site of benzyl-penicillin accumulation in Penicillium chrysogenum. Zbl Bakt II Abt 134:721–732
Kuryłowicz W, Kurz akowski W, Woźnicka W, Połowniak-Pracka H, Paszkiewicz A, Luba J, Piorunowski J (1980) Atlas of the ultrastructure of Penicillium chrysogenum in the course of biosynthesis of penicillin, Chemia, Warsaw
Kurz atkowski W, Kuryłowicz W, Paszkiewicz A (1982) Penicillin G production by immobilized fungal vesicles. Eur J Appl Microbiol Biotechnol 15:211–213
Larreta Garde V, Thomasset B, Tanaka A, Gellf G, Thomas D (1981) Comparative stabilization of biological photosystems by several immobilization procedures. 1. ATP production by immobilized bacterial chromophores. Eur J Appl Microbiol Biotechnol 11:133–138
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–267
Morikawa Y, Karube I, Suzuki S (1979) Penicillin G production by immobilized whole cells of Penicillium chrysogenum. Biotechnol Bioeng 21:261–270
Neuss N, Nash CH, Baldwin JE, Lemke PA, Grutzner JB (1973) Incorporation of (2RS, 3S)-[4-13C]-valine into cephalosporin C. J Am Chem Soc 95:3797–3798
Ohlson S, Larsson PO, Mosbach K (1979) Steroid transformation by living cells immobilized in calcium alginate. Eur J Appl Microbiol Biotechnol 7:103–110
Thomas R (1961) Colorimetric detection of penicillins and cephalosporins on paper. Nature 191:1161–1163
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Kurzakowski, W., Kuryłowicz, W. & Penyige, A. Radioactive penicillin G production by immobilized fungal vesicles. Appl Microbiol Biotechnol 19, 312–315 (1984). https://doi.org/10.1007/BF00253777
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DOI: https://doi.org/10.1007/BF00253777