Skip to main content
Log in

Localization, sequence and expression of the gene coding for tRNAPro (UGG) in plant mitochondria

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The four Sal I fragments of wheat mitochondrial DNA containing the 18S and 5S ribosomal RNA genes were screened for the presence of tRNA genes. Upon sequencing, a tRNAPro (UGG) gene was found in two of these four fragments. The localization of the corresponding gene on the maize mitochondrial genome was established. Transcriptional studies have shown that this gene is transcribed in wheat and maize mitochondria. The sequence of the corresponding tRNAPro (UGG) of bean mitochondria was determined using in vitro post-labeling techniques.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Lonsdale DM, Hodge TP, Fauron CMR: The physical map and organisation of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12: 9249–9261 (1984).

    Google Scholar 

  2. Kemble RJ, Bedbrook JR: Low molecular weight circular and linear DNA in mitochondria from normal and malesterile Zea mays cytoplasm. Nature 284: 565–566 (1980).

    Google Scholar 

  3. Palmer JD, Shields CR: Tripartite structure of Brassica campestris mitochondrial genome. Nature 307: 436–440 (1984).

    Google Scholar 

  4. Ward BL, Anderson RS, Bendich AJ: The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae). Cell 25: 793–803 (1981).

    Google Scholar 

  5. Anderson S, Bankier AT, Barrell BG, deBruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG: Sequence and organization of the human mitochondrial genome. Nature 290: 457–465 (1981).

    Google Scholar 

  6. Dujon B: Mitochondrial genes, mutants and maps: a review. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983. Walter de Gruyter, Berlin, New York, pp 1–24 (1983).

    Google Scholar 

  7. Gray MW, Spencer DF: Wheat mitochondrial DNA encodes a eubacterial-like initiator methionine transfer RNA. FEBS Lett 161: 323–327 (1983).

    Google Scholar 

  8. Parks TD, Dougherty WG, Levings CSIII, Timothy DH: Identification of two methionine transfer RNA genes in the maize mitochondrial genome. Plant Physiol 76: 1079–1082 (1984).

    Google Scholar 

  9. Parks TD, Dougherty WG, Levings CSIII, Timothy DH: Identification of an aspartate transfer RNA gene in maize mitochondrial DNA. Current Genetics 9: 517–519 (1985).

    Google Scholar 

  10. Marechal L, Guillemaut P, Grienenberger JM, Jeannin G, Weil JH: Sequence and codon recognition of bean mitochondria and chloroplast tRNAsTrp: evidence for a high degree of homology. Nucleic Acids Res 13: 4411–4416 (1985).

    Google Scholar 

  11. Lonsdale DM: Movement of genetic material between the chloroplast and mitochondrion in higher plants. In: Hohn B, Dennis ES (eds) Plant Gene Research, Vol. 2, Springer-Verlag, Vienna, New York, pp 51–60 (1985).

    Google Scholar 

  12. Stern DB, Lonsdale DM: Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299: 698–702 (1982).

    Google Scholar 

  13. Falconet D, Lejeune B, Quetier F, Gray MW: Evidence for homologous recombination between repeated sequences containing 18S and 5S ribosomal RNA genes in wheat mitochondrial DNA. EMBO J 3: 297–302 (1984).

    Google Scholar 

  14. Marechal L, Runeberg-Roos P, Grienenberger JM, Colin J, Weil JH, Lejeune B, Quetier F, Lonsdale DM: Homology in the region containing a tRNATrp gene and a (complete or partial) tRNAPro gene in wheat mitochondrial and chloroplast genomes. Curr Genet 12: 92–98 (1987).

    Google Scholar 

  15. Marechal L, Wintz H, Grienenberger JM, Guillemaut P, Jeannin G, Weil JH, Lonsdale D: Transfer RNAs of higher plant mitochondria: gene localization and sequence determination. In: Quagliarello E, Slater EC, Palmieri F, Saccone C, Kroon AM (eds), Achievements and Perspectives of Mitochondrial Research. Elsevier Science Publishers, Amsterdam (1987) pp. 122–132.

    Google Scholar 

  16. Silberklang MN, Gillum AM, RajBhandary UL: Use of in vitro 32P-labeling in the sequence analysis of non radioactive tRNAs. In: Moldave K, Grossman L (eds), Methods in Enzymology, Vol. 49, Academic Press, New York, pp 58–109 (1979).

    Google Scholar 

  17. Marechal L, Guilemaut P, Grienenberger JM, Jeannin G, Weil JH: Structure of bean mitochondrial tRNAPhe and localization of the tRNAPhe gene on the mitochondrial genomes of maize and wheat. FEBS Lett 184: 289–293 (1985).

    Google Scholar 

  18. Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF: In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular Form and Function of the Plant Genomes. NATO ASI Series A: Life Sciences 83, pp 413–420 (1985).

  19. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    Google Scholar 

  20. Wallace RB, Shaffer J, Murphy RF, Bonner J, Hirose T, Itakura K: Hybridization of synthetic oligodeoxyribonucleotides to ϕ X174 DNA: the effect of single base pair mismatch. Nucleic Acids Res 6: 3543–3557 (1979).

    Google Scholar 

  21. Stern DB, Newton KJ: Isolation of intact plant mitochondrial RNA using aurintricarboxylic acid. Plant Mol Biol Rep 2: 8–15 (1984).

    Google Scholar 

  22. Marechal L, Guillemaut P, Weil JH: Sequence of two bean mitochondria tRNAsTyr which differ in the level of posttranscriptional modification and have a prokaryotic-like large extra-loop. Plant Mol Biol 5: 347–351 (1985).

    Google Scholar 

  23. Marechal L, Guillemaut P, Grienenberger JM, Jeannin G, Weil JH: Sequences of initiator and elongator methionine tRNAs in bean mitochondria. Plant Mol Biol 7: 245–253 (1986).

    Google Scholar 

  24. Green GA, Weil JH, Steinmetz A: The sequence of two nuclear genes and a pseudogene for tRNAPro from the higher plant Phaseolus vulgaris. Plant Mol Biol 7: 207–212 (1986).

    Google Scholar 

  25. Lagerkvist U: “Two out of three”: an alternative method for codon reading. Proc Natl Acad Sci USA 75: 1759–1762 (1978).

    Google Scholar 

  26. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimida H, Sugiura M: The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J 5: 2043–2049 (1986).

    Google Scholar 

  27. Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H: Chloroplast gene organization deduced from complete sequence of liverworth Marchantia polymorpha chloroplast DNA. Nature 322: 572–574 (1986).

    Google Scholar 

  28. Stanley J, Vassilenko S: A different approach to RNA sequencing. Nature 274: 87–89 (1978).

    Google Scholar 

  29. Sprinzl M, Moll J, Meissner F, Hartmann T: Compilation of tRNA sequences. Nucleic Acids Res 13, Suppl. r1-r49 (1985).

    Google Scholar 

  30. Sprinzl M, Vorderwulbecke T, Hartmann T: Compilation of sequences of tRNA genes. Nucleic Acids Res 13, Suppl. r51-r106 (1985).

    Google Scholar 

  31. Francis M, Kashdan M, Sprouse H, Otis L, Dudock B: Nucleotide sequence of a spinach chloroplast proline tRNA. Nucleic Acids Res 10: 2755–2758 (1982).

    Google Scholar 

  32. Sibler AP, Dirheimer G, Martin RP: Codon reading pattern in Saccharomyces cerevisiae mitochondria based on sequences of mitochondrial tRNAs. FEBS Lett 194: 131–138 (1986).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Runeberg-Roos, P., Grienenberger, J.M., Guillemaut, P. et al. Localization, sequence and expression of the gene coding for tRNAPro (UGG) in plant mitochondria. Plant Mol Biol 9, 237–246 (1987). https://doi.org/10.1007/BF00166460

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00166460

Key words

Navigation