Summary
Two bean mitochondrial tRNAsTyr purified by RPC-5 chromatography and two-dimensional gel electrophoresis have been sequenced using post-labeling techniques. These two tRNAs only differ by three post-transcriptional modifications in the D-loop. They have a large variable loop and therefore resemble prokaryotic tRNAsTyr rather than eukaryotic cytoplasmic tRNAsTyr.
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Abbreviations
- D:
-
dihydrouridine
- Gm:
-
2′ O-methylguanosine
- m2G:
-
2-methylguanosine
- i6A:
-
N6 isopentenyladenosine
- ms2i6A:
-
2-methylthio N6 isopentenyladenosine
- T:
-
ribothymidine
- ψ:
-
pseudouridine
- Q:
-
queuosine
References
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Maréchal, L., Guillemaut, P. & Weil, JH. Sequences of two bean mitochondria tRNAsTyr which differ in the level of post-transcriptional modification and have a prokaryotic-like large extra-loop. Plant Mol Biol 5, 347–351 (1985). https://doi.org/10.1007/BF00037555
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DOI: https://doi.org/10.1007/BF00037555