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Isolation of Transposon Induced Auxin-Negative Mutations in Azospirillum Lipoferum

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Azospirillum IV

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Summary

A highly effective method for transposon Tn5 mutagenesis in A. lipoferum is reported. Tn5 was delivered to A. lipoferum using the suicide plasmid pSUP2021 and the donor strain E. coli SM10. Neomycin-resistant transconjugants were detected at a frequency of 6x10−6/recipient. Different types of mutants were isolated, e.g. auxotrophs, coloured, IAA-negative, and IAA-overproducers. Two hot spots were found in cysteine and methionine biosynthesis genes. Tn5-induced mutants were found to be relatively stable, with reversion rates of 2–20 × 10−8. A gene which is a part of the carotenoid pathway is closely linked to the histidine genes.

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Author information

Author notes

  1. Mohammed Safwat Abdel-Salam

    Present address: Gene-Technology Lab., National Research Centre, El-Tahrir Street, Dokki, Cairo, Egypt

Authors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, D-8580, Bayreuth, Federal Republic of Germany

    Mohammed Safwat Abdel-Salam & Walter Klingmüller

Authors
  1. Mohammed Safwat Abdel-Salam
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  2. Walter Klingmüller
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

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© 1988 Springer-Verlag Berlin Heidelberg

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Abdel-Salam, M.S., Klingmüller, W. (1988). Isolation of Transposon Induced Auxin-Negative Mutations in Azospirillum Lipoferum. In: Klingmüller, W. (eds) Azospirillum IV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73072-6_6

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  • DOI: https://doi.org/10.1007/978-3-642-73072-6_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73074-0

  • Online ISBN: 978-3-642-73072-6

  • eBook Packages: Springer Book Archive

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