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Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion

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Sorghum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1931))

Abstract

Marker assisted selection (MAS), an advance tool in plant breeding that allows accurate and efficient introgression of important agronomic trait(s) from a germplasm source to desired elite lines, has been applied to sorghum recently. Here, we report the methods for the deployment of MAS for trait introgression using endpoint genotyping technology for single nucleotide polymorphism (SNP)/insertion deletion (InDel) coupled with an application of KASP (Kompetitive Allele Specific Polymerase Chain Reaction [PCR]) chemistry allowing for the selection of parents for generational advancement without going through the laborious and time consuming phenotypic selection and additional generations for selection of desired individuals. This MAS-SNP marker assisted backcrossing scheme can be applied to accurately select heterozygotes for use as an allele donor parent in each backcross generation, thus expediting the backcrossing scheme and resulting in time savings of 3 years compared to conventional methods of introgression practiced in sorghum breeding and improvement.

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Acknowledgment

The authors gratefully acknowledge the support of US Sorghum Checkoff Program for funding the research described in this chapter.

Disclaimer: Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Plant Stress and Germplasm Development Unit, Cropping Systems Research Laboratory, USDA-ARS, Lubbock, TX, USA

    Gloria Burow, Ratan Chopra, Halee Hughes, Zhanguo Xin & John Burke

  2. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, USA

    Ratan Chopra

Authors
  1. Gloria Burow
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  2. Ratan Chopra
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  3. Halee Hughes
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  4. Zhanguo Xin
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  5. John Burke
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Corresponding author

Correspondence to Gloria Burow .

Editor information

Editors and Affiliations

  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Zuo-Yu Zhao

  2. UC-ANR Kearney Agricultural Research and Extension (KARE) Center, Parlier, CA, USA

    Jeff Dahlberg

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Burow, G., Chopra, R., Hughes, H., Xin, Z., Burke, J. (2019). Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion. In: Zhao, ZY., Dahlberg, J. (eds) Sorghum. Methods in Molecular Biology, vol 1931. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9039-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9039-9_6

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9038-2

  • Online ISBN: 978-1-4939-9039-9

  • eBook Packages: Springer Protocols

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