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Membrane Protein Production in Lactococcus lactis for Functional Studies

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Heterologous Expression of Membrane Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1432))

Abstract

Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.

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Acknowledgments

This study received financial support from the Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA), the Centre National de la Recherche Scientifique (CNRS), the French National Institute for Agricultural Research (INRA), the University Joseph Fourier (Grenoble), and the Medical Research Council UK. E.S. was funded by a joint grant from the GRAL Labex (Grenoble Alliance for Integrated Structural Cell Biology: ANR-10-LABEX-04) and the CEA.

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France

    Daphne Seigneurin-Berny, Emiline Sautron, Lucas Moyet & Norbert Rolland

  2. Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK

    Martin S. King & Edmund R. S. Kunji

  3. Laboratoire de Chimie et Biologie des Métaux, CNRS (UMR-5249)/CEA/Université Grenoble Alpes, BIG, CEA, Grenoble, France

    Patrice Catty

  4. Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris Saclay, Gif-sur-Yvette, France

    François André & Annie Frelet-Barrand

  5. FEMTO-ST Institute, UMR CNRS 6174, University of Bourgogne Franche-Comte, Besançon, France

    Annie Frelet-Barrand

Authors
  1. Daphne Seigneurin-Berny
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  2. Martin S. King
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  3. Emiline Sautron
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  4. Lucas Moyet
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  5. Patrice Catty
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  6. François André
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  7. Norbert Rolland
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  8. Edmund R. S. Kunji
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  9. Annie Frelet-Barrand
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Corresponding author

Correspondence to Annie Frelet-Barrand .

Editor information

Editors and Affiliations

  1. Université Côte d’Azur CNRS, IPMC, Valbonne, France

    Isabelle Mus-Veteau

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Seigneurin-Berny, D. et al. (2016). Membrane Protein Production in Lactococcus lactis for Functional Studies. In: Mus-Veteau, I. (eds) Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, vol 1432. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3637-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3637-3_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3635-9

  • Online ISBN: 978-1-4939-3637-3

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