Abstract
Retrozymes are a novel family of non-autonomous retrotransposable elements that contain hammerhead ribozyme motifs. These retroelements are found widespread in eukaryotic genomes, with active copies present in many species, which rely on other autonomous transposons for mobilization. Contrary to other retrotransposons, transcription of retrozymes in vivo leads to the formation and accumulation of circular RNAs, which can be readily detected by RNA blotting. In this chapter, we describe the procedures needed to carry out the cloning of genomic retrozymes, and to detect by northern blot their circular RNA retrotransposition intermediates.
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Acknowledgements
We wish to thank María Pedrote for her excellent technical assistance. This work was funded by the Ministerio de Ciencia, Innovación y Universidades of Spain and FEDER (grant BFU2017-87370-P).
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Cervera, A., de la Peña, M. (2021). Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates. In: Scarborough, R.J., Gatignol, A. (eds) Ribozymes. Methods in Molecular Biology, vol 2167. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0716-9_3
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DOI: https://doi.org/10.1007/978-1-0716-0716-9_3
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