Abstract
A stainless steel paper-embedded biofilm reactor (PEBR) was developed for Candida spp. growth, permitting confluent distribution of nutrients by capillary diffusion through ordinary laboratory filter paper. Antibiogram disks were distributed along the filter paper rim, and the PEBR received 0.1 or 0.01 % crystal violet (CV) at 200 μL min−1 and at 37 °C, for 48 h. CV was recovered from the disks and measured at 540 nm. Candida albicans SC5314 cells were applied onto antibiogram disks. The bioreactor was assembled, and YEPD broth was admitted (200 μL min−1) at 37 °C, for 72 h. Biofilm growth was estimated via the MTT reduction test. Controls were disks that received the same treatments, except for the fungus. The PEBR was considered high-throughput table, low-cost, and feasible to grow C. albicans biofilms.
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Acknowledgments
This study was granted by the Brazilian research agency Araucaria Foundation (Proc. 11524, Conv. 416/2009). Authors thank the cooperation of professors and technicians from the Electron Microscopy Centre and the Confocal Laboratory Facility of Federal University of Paraná.
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Selow, M.L.C., Rymovicz, A.U.M., Ribas, C.R. et al. Growing Candida albicans Biofilms on Paper Support and Dynamic Conditions. Mycopathologia 180, 27–33 (2015). https://doi.org/10.1007/s11046-015-9889-y
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DOI: https://doi.org/10.1007/s11046-015-9889-y