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Internal Extracellular Bacteria of Diaphorina citri Kuwayama (Hemiptera: Psyllidae), the Asian Citrus Psyllid

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Abstract

The Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an invasive insect pest that transmits Candidatus Liberibacter spp. This insect/pathogen system was first identified in North America in the early 2000’s and has become the top threat to the citrus industry. Limited options for management of this problem exist; therefore, innovative pest management strategies are being developed. In this study, we describe the first step toward a paratransgenic approach (also referred to symbiotic control) for control of the insect vector or the pathogen. Culturable bacteria from the gut of Asian Citrus Psyllids were identified using standard culture techniques followed by sequencing of the cultured microorganisms. Further, 454 pyrosequencing of the gut was performed to audit bacterial presence in order to begin to identify any relationship between psyllid symbionts and C. Liberibacter spp.

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Acknowledgments

This work was supported in part by a California State University Center for Research Student Award. This manuscript does not contain clinical studies or patient data.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd., Hayward, CA, 94543, USA

    Lakshmi D. Kolora & C. R. Lauzon

  2. United States Department of Agriculture, Agricultural Research Service, U.S. Horticultural Laboratory, Fort Pierce, FL, 34945, USA

    W. Hunter

  3. Department of Biology, University of Texas, Tyler, Tyler, 3900 University Blvd., Tyler, TX, 75799, USA

    Christopher M. Powell & B. Bextine

Authors
  1. Lakshmi D. Kolora
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  2. Christopher M. Powell
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  5. C. R. Lauzon
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Correspondence to C. R. Lauzon.

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Kolora, L.D., Powell, C.M., Hunter, W. et al. Internal Extracellular Bacteria of Diaphorina citri Kuwayama (Hemiptera: Psyllidae), the Asian Citrus Psyllid. Curr Microbiol 70, 710–715 (2015). https://doi.org/10.1007/s00284-015-0774-1

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  • DOI: https://doi.org/10.1007/s00284-015-0774-1

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