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Flavoprotein oxidases: classification and applications

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Abstract

This review provides an overview of oxidases that utilise a flavin cofactor for catalysis. This class of oxidative flavoenzymes has shown to harbour a large number of biotechnologically interesting enzymes. Applications range from their use as biocatalysts for the synthesis of pharmaceutical compounds to the integration in biosensors. Through the recent developments in genome sequencing, the number of newly discovered oxidases is steadily growing. Recent progress in the field of flavoprotein oxidase discovery and the obtained biochemical knowledge on these enzymes are reviewed. Except for a structure-based classification of known flavoprotein oxidases, also their potential in recent biotechnological applications is discussed.

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Acknowledgements

M.W. Fraaije and A. Mattevi received support from the European Union's Seventh Framework Programme (FP7/2007-2013), project Oxygreen (grant agreement no. 212281).

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Authors and Affiliations

  1. Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands

    Willem P. Dijkman, Gonzalo de Gonzalo & Marco W. Fraaije

  2. Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy

    Andrea Mattevi

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  1. Willem P. Dijkman
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  2. Gonzalo de Gonzalo
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  3. Andrea Mattevi
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  4. Marco W. Fraaije
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Correspondence to Marco W. Fraaije.

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Dijkman, W.P., de Gonzalo, G., Mattevi, A. et al. Flavoprotein oxidases: classification and applications. Appl Microbiol Biotechnol 97, 5177–5188 (2013). https://doi.org/10.1007/s00253-013-4925-7

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