Overview
- Offers cutting edge methods to analyse microRNA expression, function and post-transcriptional modifications
- Includes methods to isolate and manipulate cells of the immune system
- Provides a description of bioinformatics databases on miRNAs and their targets, as well as instructions to navigate them
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 667)
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Table of contents (21 protocols)
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Analysis of miRNA Expression: Classic Methods Revisited
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Functional Analysis of miRNAs in the Immune System: Gain-of-Function
Keywords
About this book
Reviews
From the reviews:
“This book is useful to scientists seeking to study the role of miRNAs in the immune system. … helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. … The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students.” (Elizabeth S. Raveche, Doody’s Review Service, February, 2011)
“Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field … and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. … appealing to anyone from beginners to experts. … an attractive book for those interested in identifying and characterising particular miRNAs in the immune system.” (Daniel Peters, Immunology News, February, 2011)
Editors and Affiliations
Bibliographic Information
Book Title: MicroRNAs and the Immune System
Book Subtitle: Methods and Protocols
Editors: Silvia Monticelli
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-811-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science + Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-810-2Published: 13 September 2010
Softcover ISBN: 978-1-4939-6150-4Published: 23 August 2016
eBook ISBN: 978-1-60761-811-9Published: 09 September 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 330
Topics: Immunology