MicroRNAs and the Immune System pp 165-176 | Cite as
Regulation of Monocytopoiesis by MicroRNAs
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Abstract
MicroRNAs (miRNAs or miRs) are ∼22 nt single-stranded noncoding RNAs that control gene expression in eukaryotes. miRNAs play an essential role in all basic cellular processes including cell development, proliferation, differentiation, and apoptosis. Importantly, miRNAs regulate hematopoietic progenitor cells differentiation toward the different hematopoietic lineages. This occurs through the regulation of key factors involved in hematopoiesis (e.g., transcription factors, growth factor receptors). We, hereby, describe how to investigate the role of miRNAs in monocytopoiesis.
Keywords
Flt3 Ligand Miltenyi Biotech Ammonium Chloride Solution Monocytic Differentiation Centrifuge CellNotes
Acknowledgments
This work was supported by the Italy–USA Oncology Program and the Biotechnology Program, Istituto Superiore di Sanità, and Associazione Italiana per la Ricerca sul Cancro (AIRC) to C.P.
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