Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1870)
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Table of contents (21 protocols)
Keywords
About this book
This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more aboutthis field.
Editors and Affiliations
Bibliographic Information
Book Title: Epitranscriptomics
Book Subtitle: Methods and Protocols
Editors: Narendra Wajapeyee, Romi Gupta
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8808-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8807-5Published: 12 December 2018
eBook ISBN: 978-1-4939-8808-2Published: 11 December 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 298
Number of Illustrations: 16 b/w illustrations, 41 illustrations in colour
Topics: Human Genetics