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Identification of Methylated Transcripts Using the TRIBE Approach

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Epitranscriptomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1870))

Abstract

m6A is the most abundant internal modification on mRNA. Recent improvements of high-throughput sequencing techniques enables its detection at the transcriptome level, even at the nucleotide resolution. However most current techniques require large amounts of starting material to detect the modification. Here, we describe a complementary technique of standard meRIP-seq/miCLIP-seq approaches to identify methylated RNA using a low amount of material. We believe this approach can be applied in vivo to identify methylated targets in specific tissues or subpopulations of cells.

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Acknowledgments

We thank members of the Dieterich and Roignant labs for their helpful comments and support. JYR was supported by the Deutsche Forschungsgemeinschaft (DFG) RO 4681/5-1, SPP1784 (RO 4681/9-1) and the Epitran COST action (CA16120). CD was supported by the DFG SPP1738 (DI 1501/5-1) and SPP1935 (DI 1501/8-1).

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Authors and Affiliations

  1. Laboratory of RNA Epigenetics, Institute of Molecular Biology (IMB), Mainz, Germany

    Lina Worpenberg & Jean-Yves Roignant

  2. Department of Internal Medicine III, University Hospital Heidelberg, Heidelberg, Germany

    Tobias Jakobi & Christoph Dieterich

  3. DZHK (German Centre for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Heidelberg, Germany

    Tobias Jakobi & Christoph Dieterich

Authors
  1. Lina Worpenberg
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  2. Tobias Jakobi
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  3. Christoph Dieterich
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  4. Jean-Yves Roignant
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Corresponding authors

Correspondence to Christoph Dieterich or Jean-Yves Roignant .

Editor information

Editors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Narendra Wajapeyee

  2. Yale Cancer Center, Yale University School of Medicine, New Haven, CT, USA

    Romi Gupta

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Worpenberg, L., Jakobi, T., Dieterich, C., Roignant, JY. (2019). Identification of Methylated Transcripts Using the TRIBE Approach. In: Wajapeyee, N., Gupta, R. (eds) Epitranscriptomics. Methods in Molecular Biology, vol 1870. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8808-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8808-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8807-5

  • Online ISBN: 978-1-4939-8808-2

  • eBook Packages: Springer Protocols

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