Abstract
The mammalian nervous system consists of neurons that transmit the coordinated neuronal signals throughout the body and glia or supporting cells that support, protect, and nourish the neurons. Adult neurons lose the capacity to divide due to the absence of centrioles. The supporting or glia cells which are around ten times in number than neuronal cells continuously divide throughout the lifetime. However, new neurons arise from neural stem cells (NSCs) during embryogenesis and are preserved even in adults within the specified brain regions such as the subventricular zone and other sections of the nervous system. Additionally, neuronal cell lines are derived from the neuronal tumor/cancer cells that have a capacity for continuous divisions. Immortal cell lines are characteristically different than NSCs. The present chapter describes the basics of isolation and primary culture of neuronal cells, particularly NSCs from various brain regions such as the hippocampus, striatum, and cerebellum. The culturing methods of primary neuronal stem cells and various neurological cell lines such as PC-12 and HEK-293 are discussed. Additionally, isolation and culture of glia or supporting cells, particularly microglial cell culture, have been included.
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Mukherjee, S., Malik, P., Mukherjee, T.K. (2023). Culture of Neuron and Glia Cells. In: Mukherjee, T.K., Malik, P., Mukherjee, S. (eds) Practical Approach to Mammalian Cell and Organ Culture. Springer, Singapore. https://doi.org/10.1007/978-981-19-1731-8_10-2
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DOI: https://doi.org/10.1007/978-981-19-1731-8_10-2
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Latest
Culture of Neuron and Glia Cells- Published:
- 20 January 2023
DOI: https://doi.org/10.1007/978-981-19-1731-8_10-2
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Culture of Neuron and Glia Cells- Published:
- 27 December 2022
DOI: https://doi.org/10.1007/978-981-19-1731-8_10-1