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Gas Chromatography of Fatty Acid Methyl Esters: Resolution Using Conventional and High-Resolution Columns

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Encyclopedia of Lipidomics

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Synonyms

GC; FAME; GC columns

No single method is currently available for separate fatty acids from complex mixture of natural (e.g., animal tissues such as milk, muscle, adipose tissue, or plasma) or modified lipids. However, a set of comprehensive analytical techniques could maximize the separation and quantification of most of the fatty acids. GC remains by far the most convenient method to identify and quantify fatty acid methyl esters (FAME). The nature of the stationary phase, column internal diameter, film thickness, and column length are important factors to select a proper column for specific application. Polar stationary phases are recommended for the separation of FAME. Packed and capillary (also known as open tubular) are the two main categories of columns. Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase. Most packed columns are 1.5–10 m in length and have an internal diameter...

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Authors and Affiliations

  1. Nestlé Research Center, Nestec Ltd, Vers-chez-les-Blanc, 26, P.O. Box 44, 1000, Lausanne, Switzerland

    Cristina Cruz-Hernandez & Frédéric Destaillats

Authors
  1. Cristina Cruz-Hernandez
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  2. Frédéric Destaillats
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Correspondence to Cristina Cruz-Hernandez or Frédéric Destaillats .

Editor information

Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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Cruz-Hernandez, C., Destaillats, F. (2016). Gas Chromatography of Fatty Acid Methyl Esters: Resolution Using Conventional and High-Resolution Columns. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_103-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_103-1

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  • Online ISBN: 978-94-007-7864-1

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