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Base-catalyzed derivatization methodology for FA analysis. Application to milk fat and celery seed lipid TAG

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Lipids

Abstract

In this paper, an alternative base-catalyzed methodology for the facile derivatization in mild conditions of lipid TAG prior to FA analysis is proposed. Reagents were prepared by proton exchange between potassium tert-butoxide and either ethanol, n-propanol, n-butanol, or 2-methoxyethanol and used for the synthesis, at 40°C for 15 min, of the corresponding derivatives, which were directly analyzed by GC. This methodology can be used on a routine basis and has been applied to standard and complex natural lipid samples. Tripalmitin was used to determine optimal reaction conditions; and bovine milk fat, containing C4 to C22 acids, and celery (Apium graveolens) seed oil, characterized by a high level of petroselinic acid, were comparatively analyzed as their ethyl, n-propyl, n-butyl, and 2-methoxyethyl esters.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, and Dairy Research Center (STELA), Université Laval, G1K 7P4, Sainte-Foy, Québec, Canada

    Frédéric Destaillats & Paul Angers

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  1. Frédéric Destaillats
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  2. Paul Angers
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Correspondence to Paul Angers.

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Destaillats, F., Angers, P. Base-catalyzed derivatization methodology for FA analysis. Application to milk fat and celery seed lipid TAG. Lipids 37, 527–532 (2002). https://doi.org/10.1007/s11745-002-0928-9

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  • DOI: https://doi.org/10.1007/s11745-002-0928-9

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