Skip to main content

UDP-GlcNAc: BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5, Lc3Cer Synthase)

  • Reference work entry
  • First Online:
Handbook of Glycosyltransferases and Related Genes

Abstract

β1,3-N-acetylglucosaminyltransferases (β3GnTs) synthesize a unique carbohydrate structure known as polylactosamine (poly-N-acetyllactosamine). Polylactosamines are carried on N- and O-glycans and glycolipids. The polylactosamine unit is considered an integral component that serves as a fundamental structure and backbone for carbohydrates.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 799.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 549.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Ashwell KW, Mai JK (1997) A transient CD15 immunoreactive sling in the developing mouse cerebellum. Int J Dev Neurosci 15:883–889

    Article  CAS  PubMed  Google Scholar 

  • Biellmann F, Hulsmeier AJ, Zhou D, Cinelli P, Hennet T (2008) The Lc3-synthase gene B3gnt5 is essential to pre-implantation development of the murine embryo. BMC Dev Biol 8:109

    Article  PubMed Central  PubMed  Google Scholar 

  • Chou DK, Jungalwala FB (1993a) Characterization and developmental expression of a novel sulfotransferase for the biosynthesis of sulfoglucuronyl glycolipids in the nervous system. J Biol Chem 268:330–336

    CAS  PubMed  Google Scholar 

  • Chou DK, Jungalwala FB (1993b) N-acetylglucosaminyltransferase regulates the expression of neolactoglycolipids including sulfoglucuronylglycolipids in the developing nervous system. J Biol Chem 268:21727–21733

    CAS  PubMed  Google Scholar 

  • Chou DK, Flores S, Jungalwala FB (1991) Expression and regulation of UDP-glucuronate: neolactotetraosylceramide glucuronyltransferase in the nervous system. J Biol Chem 266:17941–17947

    CAS  PubMed  Google Scholar 

  • Chou DK, Henion TR, Jungalwala FB (2003) Regulation of expression of sulfoglucuronyl carbohydrate (HNK-1), Amphoterin and RAGE in retinoic acid-differentiated P19 embryonal carcinoma cells. J Neurochem 86:917–931

    Article  CAS  PubMed  Google Scholar 

  • Etcheverry A, Aubry M, de Tayrac M, Vauleon E, Boniface R, Guenot F, Saikali S, Hamlat A, Riffaud L, Menei P, Quillien V, Mosser J (2010) DNA methylation in glioblastoma: impact on gene expression and clinical outcome. BMC Genomics 11:701

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Gocht A, Struckhoff G, Lhler J (1996) CD15-Containing glycoconjugates in the central nervous system. Histol Histopathol 11:1007–1028

    CAS  PubMed  Google Scholar 

  • Henion TR, Zhou D, Wolfer DP, Jungalwala FB, Hennet T (2001) Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis. J Biol Chem 276:30261–30269

    Article  CAS  PubMed  Google Scholar 

  • Hennet T, Dinter A, Kuhnert P, Mattu TS, Rudd PM, Berger EG (1998) Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes. J Biol Chem 273:58–65

    Article  CAS  PubMed  Google Scholar 

  • Holmes EH, Hakomori S, Ostrander GK (1987) Synthesis of type 1 and 2 lacto series glycolipid antigens in human colonic adenocarcinoma and derived cell lines is due to activation of a normally unexpressed beta 1–3N-acetylglucosaminyltransferase. J Biol Chem 262:15649–15658

    CAS  PubMed  Google Scholar 

  • Hu J, Stults CL, Holmes EH, Macher BA (1994) Structural characterization of intermediates in the biosynthetic pathway of neolacto glycosphingolipids: differential expression in human leukaemia cells. Glycobiology 4:251–257

    Article  PubMed  Google Scholar 

  • Jungalwala FB (1994) Expression and biological functions of sulfoglucuronyl glycolipids (SGGLs) in the nervous system – a review. Neurochem Res 19:945–957

    Article  CAS  PubMed  Google Scholar 

  • Kuan CT, Chang J, Mansson JE, Li J, Pegram C, Fredman P, McLendon RE, Bigner DD (2010) Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase – a key enzyme in lacto-neolacto ganglioside synthesis. BMC Dev Biol 10:114

    Article  PubMed Central  PubMed  Google Scholar 

  • Mai JK, Krajewski S, Reifenberger G, Genderski B, Lensing-Hohn S, Ashwell KW (1999) Spatiotemporal expression gradients of the carbohydrate antigen (CD15) (Lewis X) during development of the human basal ganglia. Neuroscience 88:847–858

    Article  CAS  PubMed  Google Scholar 

  • Parks JC, McCallie BR, Janesch AM, Schoolcraft WB, Katz-Jaffe MG (2011) Blastocyst gene expression correlates with implantation potential. Fertil Steril 95:1367–1372

    Article  CAS  PubMed  Google Scholar 

  • Schwarting GA (1980) Quantitative analysis of neutral glycosphingolipids from human lymphocyte subpopulations. Biochem J 189:407–412

    CAS  PubMed  Google Scholar 

  • Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K (2001) Identification and characterization of three novel β 1,3-N-acetylglucosaminyltransferases structurally related to the β 1,3-galactosyltransferase family. J Biol Chem 276:3498–3507

    Article  CAS  PubMed  Google Scholar 

  • Streit A, Yuen CT, Loveless RW, Lawson AM, Finne J, Schmitz B, Feizi T, Stern CD (1996) The Le(x) carbohydrate sequence is recognized by antibody to L5, a functional antigen in early neural development. J Neurochem 66:834–844

    Article  CAS  PubMed  Google Scholar 

  • Togayachi A, Akashima T, Ookubo R, Kudo T, Nishihara S, Iwasaki H, Natsume A, Mio H, Inokuchi J, Irimura T, Sasaki K, Narimatsu H (2001) Molecular cloning and characterization of UDP-GlcNAc:Lactosylceramide β 1,3-N-acetylglucosaminyltransferase (β 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids. J Biol Chem 276:22032–22040

    Article  CAS  PubMed  Google Scholar 

  • Togayachi A, Sato T, Narimatsu H (2006) Comprehensive enzymatic characterization of glycosyltransferases with a β3GT or β4GT motif. Methods Enzymol 416:91–102

    Article  CAS  PubMed  Google Scholar 

  • Togayachi A, Kozono Y, Ikehara Y, Ito H, Suzuki N, Tsunoda Y, Abe S, Sato T, Nakamura K, Suzuki M, Goda HM, Ito M, Kudo T, Takahashi S, Narimatsu H (2010) Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation. Proc Natl Acad Sci USA 107:11900–11905

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yohe HC, Coleman DL, Ryan JL (1985) Ganglioside alterations in stimulated murine macrophages. Biochim Biophys Acta 818:81–86

    Article  CAS  PubMed  Google Scholar 

  • Zhou D, Dinter A, Gutierrez Gallego R, Kamerling JP, Vliegenthart JF, Berger EG, Hennet T (1999) A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases. Proc Natl Acad Sci USA 96:406–411

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Akira Togayachi .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Japan

About this entry

Cite this entry

Togayachi, A., Narimatsu, H. (2014). UDP-GlcNAc: BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5, Lc3Cer Synthase). In: Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (eds) Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54240-7_91

Download citation

Publish with us

Policies and ethics