Replication Licensing System
The “Replication Licensing System” is critical for the maintenance of genome stability by ensuring that no section of chromosomal genomic DNA is replicated more than once within a single cell division cycle (Arias and Walter 2007; Blow and Dutta 2005; Blow and Gillespie 2008). In late M and G1 phase, but not at other stages of the cell cycle, replication origins are licensed for a single initiation event in the upcoming S phase by encircling DNA with double hexamers of the minichromosome maintenance proteins, MCM2–7 (Arias and Walter 2007; Blow and Dutta 2005; Blow and Gillespie 2008; Deegan and Diffley 2016; Riera et al. 2017). During S phase of the cell cycle, DNA-bound MCM2–7 are activated to form the primary replicative helicase that unwinds the DNA ahead of each replication fork (Deegan and Diffley 2016; Riera et al. 2017). When replication forks terminate, MCM2–7 are removed from the DNA, thus ensuring that no section of the genome is rereplicated in a single cell...
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